Proteomic analysis of calcium-enriched sol–gel biomaterials
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Otros documentos de la autoría: Romero-Gavilán, Francisco J; Gomes, Nuno; Cerqueira, Andreia; García-Arnáez, Iñaki; Martinez Ramos, Cristina; Azkargorta, Mikel; Iloro, Ibon; Elortza, Felix; GURRUCHAGA, MARILO; Suay, Julio; Goñi, Isabel
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
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INVESTIGACIONMetadatos
Título
Proteomic analysis of calcium-enriched sol–gel biomaterialsAutoría
Fecha de publicación
2019-04Editor
SpringerCita bibliográfica
Romero-Gavilán, F., Araújo-Gomes, N., Cerqueira, A. et al. J Biol Inorg Chem (2019). https://doi.org/10.1007/s00775-019-01662-5Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://link.springer.com/article/10.1007/s00775-019-01662-5#enumerationVersión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at ... [+]
Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at the tissue–material interface, affecting the protein deposition process and cell responses. The aim of this study was to analyze the changes in the patterns of protein adsorption on the silica hybrid biomaterials supplemented with different amounts of CaCl2, which can function as release vehicles. This characterization was carried out by incubating the Ca-biomaterials with human serum. LC–MS/MS analysis was used to characterize the adsorbed protein layers and compile a list of proteins whose affinity for the surfaces might depend on the CaCl2 content. The attachment of pro- and anti-clotting proteins, such as THRB, ANT3, and PROC, increased significantly on the Ca-materials. Similarly, VTNC and APOE, proteins directly involved on osteogenic processes, attached preferentially to these surfaces. To assess correlations with the proteomic data, these formulations were tested in vitro regarding their osteogenic and inflammatory potential, employing MC3T3-E1 and RAW 264.7 cell lines, respectively. The results confirmed a Ca dose-dependent osteogenic and inflammatory behavior of the materials employed, in accordance with the protein attachment patterns. [-]
Proyecto de investigación
MINECO [MAT2017-86043-R]; Universitat Jaume I [Grant numbers Predoc/2014/25, UJI-B2017-37]; Basque Government [Grant numbers IT611-13, Predoc/2016/1/0141]; University of the Basque Country [Grant number UFI11/56] ; ProteoRed-ISCIII (Grant PRB3 IPT17/0019); CIBERehd Network, and Severo Ochoa Grant (SEV-2016-0644)Derechos de acceso
© Society for Biological Inorganic Chemistry (SBIC) 2019
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info:eu-repo/semantics/openAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
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