Browsing UJI: Investigación by Author "9e42df61-732d-4b36-a53c-06ca963b8b71"
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Biphasic concentration patterns in ionic transport under nanoconfinement revealed in steady-state and time-dependent properties
Queralt-Martín, María; Perez-Grau, Jose J.; Alvero González, Laidy Maidel; Perini, Deborah Aurora; Cervera, Javier; Aguilella, Vicente; Alcaraz, Antonio American Institute of Physics (2023-02-08)Ion permeation across nanoscopic structures differs considerably from microfluidics because of strong steric constraints, transformed solvent properties, and charge-regulation effects revealed mostly in diluted solutions. ... -
Boron clusters (ferrabisdicarbollides) shaping the future as radiosensitizers for multimodal (chemo/radio/PBFR) therapy of glioblastoma
Nuez Martínez, Miquel; Queralt-Martín, María; Aguilella, Vicente; VV.AA. Royal Society of Chemistry (2022-10-24)Glioblastoma multiforme (GBM) is the most common and fatal primary brain tumor, and is highly resistant to conventional radiotherapy and chemotherapy. Therefore, the development of multidrug resistance and tumor recurrence ... -
Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus
To, Janet; Surya, Wahyu; To, Sing Fung; Li, Yan; Verdiá Báguena, Carmen; Queralt-Martín, María; Aguilella, Vicente; Ding, Xiang Liu; Torres, Jaume American Society for Microbiology (2016-12)It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity ... -
Divalent cations reduce the pH sensitivity of OmpF channel inducing the pKa shift of key acidic residues
Queralt-Martín, María; Alcaraz, Antonio; García Giménez, Elena; Mafe, Salvador RSC Publishing (2010-11-03)In contrast to the highly-selective channels of neurophysiology employing mostly the exclusion mechanism, different factors account for the selectivity of large channels. Elucidation of these factors is essential for ... -
Dynorphin A induces membrane permeabilization by formation of proteolipidic pores. Insights from electrophysiology and computational simulations
Perini, Deborah Aurora; Aguilella-Arzo, Marcel; Alcaraz, Antonio; Perálvarez-Marín, Alex; Queralt-Martín, María Elsevier (2021-12-16)Dynorphins are endogenous neuropeptides that function as ligands for the κ-opioid receptor. In addition to opioid activity, dynorphins can induce several pathological effects such as neurological dysfunctions and cell ... -
Electrical pumping of potassium ions against an external concentration gradient in a biological ion channel
Aguilella, Vicente; Alcaraz, Antonio; Ramírez, Patricio; García Giménez, Elena; Mafe, Salvador; Queralt-Martín, María AIP Publishing (2013-07-22)We show experimentally and theoretically that significant currents can be obtained with a biological ion channel, the OmpF porin of Escherichia coli, using zero-average potentials as driving forces. The channel rectifying ... -
Entropy–enthalpy compensation at the single protein level: pH sensing in the bacterial channel OmpF
Alcaraz, Antonio; Queralt-Martín, María; Verdiá Báguena, Carmen; Aguilella, Vicente; Mafe, Salvador The Royal Society of Chemistry (2014)The pH sensing mechanism of the OmpF channel operates via ligand modification: increasing acidity induces the replacement of cations with protons in critical binding sites decreasing the channel conductance. Aside from the ... -
Excess white noise to probe transport mechanisms in a membrane channel
Queralt-Martín, María; López Peris, María Lidón; Alcaraz, Antonio American Physical Society (2015)Current fluctuation analysis has been successfully used over the years to investigate the physical properties of different systems. Here, we perform single-channel time-resolved current experiments in a protein channel ... -
Experimental demonstration of charge inversion in a protein channel in the presence of monovalent cations
López Peris, María Lidón; Queralt-Martín, María; Alcaraz, Antonio Elsevier (2014-11)Charge inversion takes place when interfacial charges attract counterions in excess of their own nominal charge. To date, this phenomenon has been reported in a variety of membrane channel systems in the presence of ... -
Fluctuation-Driven Transport in Biological Nanopores. A 3D Poisson–Nernst–Planck Study
Aguilella-Arzo, Marcel; Queralt-Martín, María; López, María Lidón; Alcaraz, Antonio MDPI (2017)Living systems display a variety of situations in which non-equilibrium fluctuations couple to certain protein functions yielding astonishing results. Here we study the bacterial channel OmpF under conditions similar to ... -
Increased salt concentration promotes competitive block of OmpF channel by protons
Alcaraz, Antonio; García Giménez, Elena; Aguilella, Vicente; Queralt-Martín, María Elsevier (2012-11)Porins are channel-forming proteins that are located in the outer membranes (OM) of Gram-negative bacteria and allow the influx of hydrophilic nutrients and the extrusion of waste products. The fine regulation of the ion ... -
Insights on the permeability of wide protein channels: measurement and interpretation of ion selectivity
Aguilella, Vicente; Queralt-Martín, María; Aguilella-Arzo, Marcel; Alcaraz, Antonio Royal Society of Chemistry (2012-10-11)Ion channels are hollow proteins that have evolved to exhibit discrimination between charged solutes. This property, known as ion selectivity is critical for several biological functions. By using the bacterial porin OmpF ... -
Ion Transport in Confined Geometries below the Nanoscale: Access Resistance Dominates Protein Channel Conductance in Diluted Solutions
Alcaraz, Antonio; López Peris, María Lidón; Queralt-Martín, María; Aguilella, Vicente (2017)Synthetic nanopores and mesoscopic protein channels have common traits like the importance of electrostatic interactions between the permeating ions and the nanochannel. Ion transport at the nanoscale occurs under confinement ... -
Lipid Headgroup Charge and Acyl Chain Composition Modulate Closure of Bacterial β-Barrel Channels
Perini, Deborah Aurora; Alcaraz, Antonio; Queralt-Martín, María MDPI (2019-02)The outer membrane of Gram-negative bacteria contains β-barrel proteins that form high-conducting ion channels providing a path for hydrophilic molecules, including antibiotics. Traditionally, these proteins have been ... -
On the different sources of cooperativity in pH titrating sites of a membrane protein channel
Alcaraz, Antonio; Queralt-Martín, María EDP Sciences (2016)Cooperative interactions play a central role in the regulation of protein functions. Here we show that in multi-site systems like ion channels the application of the Hill formalism could require a combination of different ... -
Probing electrophysiological activity of amphiphilic Dynorphin A in planar neutral membranes reveals both ion channel-like activity and neuropeptide translocation
Alvero González, Laidy Maidel; Perini, Deborah Aurora; Queralt-Martín, María; Peralvarez-Marin, Alex; Viñas, Clara; Alcaraz, Antonio Elsevier (2023-12)Dynorphin A (DynA) is an endogenous neuropeptide that besides acting as a ligand of the κ-opioid receptor, presents some non-opioid pathophysiological properties associated to its ability to induce cell permeability similarly ... -
Probing Tubulin-Blocked State of VDAC by Varying Membrane Surface Charge
Gurnev, Philip A.; Queralt-Martín, María; Aguilella, Vicente; Rostovtseva, Tatiana K. Biophysical Society (2012-05) -
Protein Ion Channels as Molecular Ratchets. Switchable Current Modulation in Outer Membrane Protein F Porin Induced by Millimolar La3+ Ions
Verdiá Báguena, Carmen; Queralt-Martín, María; Aguilella, Vicente; Alcaraz, Antonio American Chemical Society (2012)The quest for innovative tunable nanodevices has mainly focused on switches that modulate their properties through engineered conformational changes. We propose here an alternative route that takes advantage of the crucial ... -
Role of Severe Acute Respiratory Syndrome Coronavirus Viroporins E, 3a, and 8a in Replication and Pathogenesis
Castaño Rodríguez, Carlos; Honrubia, José M.; Gutiérrez-Álvarez, Javier; De Diego, Marta L.; Nieto Torres, José L.; Jiménez Guardeño, José M.; Regla Nava, José A.; Fernández Delgado, Raúl; Verdiá Báguena, Carmen; Queralt-Martín, María; Kochan, Grazyna; Perlman, Stanley; Aguilella, Vicente; Sola, Isabel; Enjuanes, Luis American Society for Microbiology (2018)Viroporins are viral proteins with ion channel (IC) activity that play an important role in several processes, including virus replication and pathogenesis. While many coronaviruses (CoVs) encode two viroporins, severe ... -
Scaling Behavior of Ionic Transport in Membrane Nanochannels
Queralt-Martín, María; López Peris, María Lidón; Aguilella-Arzo, Marcel; Aguilella, Vicente; Alcaraz, Antonio American Chemical Society (2018)Ionic conductance in membrane channels exhibits a power-law dependence on electrolyte concentration (G ∼ c α ). The many scaling exponents, α, reported in the literature usually require detailed interpretations ...