Probing electrophysiological activity of amphiphilic Dynorphin A in planar neutral membranes reveals both ion channel-like activity and neuropeptide translocation
Impacto
Scholar |
Otros documentos de la autoría: Alvero González, Laidy Maidel; Perini, Deborah Aurora; Queralt-Martín, María; Peralvarez-Marin, Alex; Viñas, Clara; Alcaraz, Antonio
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2507
comunitat-uji-handle3:10234/6973
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
Probing electrophysiological activity of amphiphilic Dynorphin A in planar neutral membranes reveals both ion channel-like activity and neuropeptide translocationAutoría
Fecha de publicación
2023-12Editor
ElsevierISSN
1567-5394Cita bibliográfica
L. M. Alvero-Gonzalez, D. A. Perini, M. Queralt-Martín, A. Perálvarez-Marín, C. Viñas, A. Alcaraz, Probing electrophysiological activity of amphiphilic Dynorphin A in planar neutral membranes reveals both ion channel-like activity and neuropeptide translocation. Bioelectrochemistry. 154 (2023) 108527, https://doi.org/10.1016/j.bioelechem.2023.108527.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.sciencedirect.com/science/article/pii/S1567539423001640Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Dynorphin A (DynA) is an endogenous neuropeptide that besides acting as a ligand of the κ-opioid receptor, presents some non-opioid pathophysiological properties associated to its ability to induce cell permeability ... [+]
Dynorphin A (DynA) is an endogenous neuropeptide that besides acting as a ligand of the κ-opioid receptor, presents some non-opioid pathophysiological properties associated to its ability to induce cell permeability similarly to cell-penetrating peptides (CPPs). Here, we use electrophysiology experiments to show that amphiphilic DynA generates aqueous pores in neutral membranes similar to those reported previously in charged membranes, but we also find other events thermodynamically incompatible with voltage-driven ion channel activity (i.e. non-zero currents with no applied voltage in symmetric salt conditions, reversal potentials that exceed the theoretical limit for a given salt concentration gradient). By comparison with current traces generated by other amphiphilic molecule known to spontaneously cross membranes, we hypothesize that DynA could directly translocate across neutral bilayers, a feature never observed in charged membranes following the same electrophysiological protocol. Our findings suggest that DynA interaction with the cellular membrane is modulated by the lipid charge distribution, enabling either passive ionic transport via membrane remodeling and pore formation or by peptide direct internalization independent of cellular transduction pathways. [-]
Publicado en
Bioelectrochemistry, 2023, vol. 154Entidad financiadora
Ministerio de Ciencia, Innovación y Universidades | Universitat Jaume I
Identificador de la entidad financiadora
http://dx.doi.org/10.13039/501100011033
Código del proyecto o subvención
MICIU/ICTI2017-2020/PID2019-108434GB-I00 | MICIU/IJC2018-035283-I | MICIU/ICTI2017-2020/PID2020-120222GB-I00 | UJI-B2022-4 | UJI-A2020-21
Título del proyecto o subvención
Estudio biofísico de los mecanismos de permeabilización de membranas inducidos por canales iónicos | Determinantes moleculares en canales iónicos: desde formación de poros a identificación de fármacos basada en ligandos
Derechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- FCA_Articles [511]