Simultaneous separation and determination of quinolones in pharmaceuticals by micellar liquid chromatography
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Other documents of the author: Collado Sánchez, María Ángeles; Rambla Alegre, María; Carda-Broch, Samuel; Esteve-Romero, Josep
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comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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Title
Simultaneous separation and determination of quinolones in pharmaceuticals by micellar liquid chromatographyAuthor (s)
Date
2010Publisher
Taylor & FrancisISSN
1082-6076Bibliographic citation
Journal of Liquid Chromatography and Related Technologies (2010), vol. 33, no. 4, p. 513-525Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/submittedVersionSubject
Abstract
A rapid and simple liquid chromatographic procedure using micellar mobile phases is reported for the separation and determination of four quinolones (pipemidic acid, levofloxacin, norfloxacin and moxifloxacin) in ... [+]
A rapid and simple liquid chromatographic procedure using micellar mobile phases is reported for the separation and determination of four quinolones (pipemidic acid, levofloxacin, norfloxacin and moxifloxacin) in pharmaceuticals.
This purpose was achieved without any previous pretreatment step in a C18 column using a micellar mobile phase of 0.15 M sodium dodecyl sulphate, 2.5% propanol and 0.5% triethylamine at pH 3, with retention times below 12 min. For detection, the diode-array UV-Vis set at 276 nm was used. The limits of detection and quantification were between 8-51 and 28-171 ng/mL, respectively. This method was validated in terms of intra-day and inter-day precision and accuracy, and robustness. Calibration curves over the concentration range of 0.1-50 μg/mL were linear (r2 > 0.9997) and. Good claim percentages (96–106 %) were obtained in the analysis of pharmaceutical formulations. The results show that the procedure is suitable for the routine analysis of drugs. [-]
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