2024-03-29T10:18:07Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/330562021-07-07T15:30:49Zcom_10234_7013com_10234_9col_10234_8638
00925njm 22002777a 4500
dc
Collado Sánchez, María Ángeles
author
Rambla Alegre, María
author
Carda-Broch, Samuel
author
Esteve-Romero, Josep
author
2010
A rapid and simple liquid chromatographic procedure using micellar mobile phases is reported for the separation and determination of four quinolones (pipemidic acid, levofloxacin, norfloxacin and moxifloxacin) in pharmaceuticals.
This purpose was achieved without any previous pretreatment step in a C18 column using a micellar mobile phase of 0.15 M sodium dodecyl sulphate, 2.5% propanol and 0.5% triethylamine at pH 3, with retention times below 12 min. For detection, the diode-array UV-Vis set at 276 nm was used. The limits of detection and quantification were between 8-51 and 28-171 ng/mL, respectively. This method was validated in terms of intra-day and inter-day precision and accuracy, and robustness. Calibration curves over the concentration range of 0.1-50 μg/mL were linear (r2 > 0.9997) and. Good claim percentages (96–106 %) were obtained in the analysis of pharmaceutical formulations. The results show that the procedure is suitable for the routine analysis of drugs.
Journal of Liquid Chromatography and Related Technologies (2010), vol. 33, no. 4, p. 513-525
1082-6076
http://hdl.handle.net/10234/33056
http://dx.doi.org/10.1080/10826070903574519
Quinolone
Fluoroquinolone
Micellar mobile phase
SDS
Direct injection
Pharmaceutical formulation
Simultaneous separation and determination of quinolones in pharmaceuticals by micellar liquid chromatography