Xanthine derivatives quantification in serum by capillary zone electrophoresis
Scholar | Otros documentos del autor: Peris Vicente, Juan; Rambla Alegre, María; Durgbanshi, Abhilasha; Bose, Devasish; Esteve-Romero, Josep; Marco Peiró, Sergio
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TítuloXanthine derivatives quantification in serum by capillary zone electrophoresis
Fecha de publicación2015-06-22
Versión del editorhttp://chromsci.oxfordjournals.org/content/52/9/1121
Otros IdentificadoresPERIS-VICENTE, Juan, et al. Xanthine Derivatives Quantification in Serum by Capillary Zone Electrophoresis. Journal of chromatographic science, 2014, 52.9: 1121-1126.
A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central ... [+]
A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75 μm (i.d.) and 61 cm (total length); absorbance detection, 280 nm; borate buffer, 20 mM, pH 9.0; applied voltage, 25 kV and 1 psi injection/8 s. Validation was performed in blank serum following the International Conference Harmonization guidelines: resolution (peaks without overlapping), linear range (0.125–50 µg/mL; r2 > 0.9999), limits of detection and quantification (10; 20 and 33; 66 ppb for caffeine and theophylline, respectively), intra- and inter-day precision (Relative standard deviation lower than 1.9%) and accuracy (98–101%). Migration times were <8 min. This method is simple, specific and suitable and reaches high label claims (98.7–100.4%) in pharmaceutical formulations analysis. Moreover, the method was applied to the monitoring of the analytes in serum of patients. [-]
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