2024-03-28T08:08:38Zhttps://repositori.uji.es/oai/requestoai:repositori.uji.es:10234/1245432023-10-31T11:07:12Zcom_10234_7013com_10234_9col_10234_8638
Repositori UJI
author
Vives-Peris, Vicente
author
Rambla Alegre, María
author
Durgbanshi, Abhilasha
author
Bose, Devasish
author
Esteve-Romero, Josep
author
Marco Peiró, Sergio
2015-06-22T07:18:15Z
2015-06-22T07:18:15Z
2015-06-22
0021-9665
PERIS-VICENTE, Juan, et al. Xanthine Derivatives Quantification in Serum by Capillary Zone Electrophoresis. Journal of chromatographic science, 2014, 52.9: 1121-1126.
http://hdl.handle.net/10234/124543
http://dx.doi.org/10.1093/chromsci/bmt164
A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75 μm (i.d.) and 61 cm (total length); absorbance detection, 280 nm; borate buffer, 20 mM, pH 9.0; applied voltage, 25 kV and 1 psi injection/8 s. Validation was performed in blank serum following the International Conference Harmonization guidelines: resolution (peaks without overlapping), linear range (0.125–50 µg/mL; r2 > 0.9999), limits of detection and quantification (10; 20 and 33; 66 ppb for caffeine and theophylline, respectively), intra- and inter-day precision (Relative standard deviation lower than 1.9%) and accuracy (98–101%). Migration times were <8 min. This method is simple, specific and suitable and reaches high label claims (98.7–100.4%) in pharmaceutical formulations analysis. Moreover, the method was applied to the monitoring of the analytes in serum of patients.
eng
© The Author [2013]. Published by Oxford University Press. All rights reserved.
Capillary electrophoresis
Xanthine derivatives quantification in serum by capillary zone electrophoresis
info:eu-repo/semantics/article
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