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Xanthine derivatives quantification in serum by capillary zone electrophoresis
dc.contributor.author | Vives-Peris, Vicente | |
dc.contributor.author | Rambla Alegre, María | |
dc.contributor.author | Durgbanshi, Abhilasha | |
dc.contributor.author | Bose, Devasish | |
dc.contributor.author | Esteve-Romero, Josep | |
dc.contributor.author | Marco Peiró, Sergio | |
dc.date.accessioned | 2015-06-22T07:18:15Z | |
dc.date.available | 2015-06-22T07:18:15Z | |
dc.date.issued | 2015-06-22 | |
dc.identifier.issn | 0021-9665 | |
dc.identifier.other | PERIS-VICENTE, Juan, et al. Xanthine Derivatives Quantification in Serum by Capillary Zone Electrophoresis. Journal of chromatographic science, 2014, 52.9: 1121-1126. | |
dc.identifier.uri | http://hdl.handle.net/10234/124543 | |
dc.description.abstract | A capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75 μm (i.d.) and 61 cm (total length); absorbance detection, 280 nm; borate buffer, 20 mM, pH 9.0; applied voltage, 25 kV and 1 psi injection/8 s. Validation was performed in blank serum following the International Conference Harmonization guidelines: resolution (peaks without overlapping), linear range (0.125–50 µg/mL; r2 > 0.9999), limits of detection and quantification (10; 20 and 33; 66 ppb for caffeine and theophylline, respectively), intra- and inter-day precision (Relative standard deviation lower than 1.9%) and accuracy (98–101%). Migration times were <8 min. This method is simple, specific and suitable and reaches high label claims (98.7–100.4%) in pharmaceutical formulations analysis. Moreover, the method was applied to the monitoring of the analytes in serum of patients. | ca_CA |
dc.format.extent | 16 p. | ca_CA |
dc.language.iso | eng | ca_CA |
dc.relation.isPartOf | Journal of chromatographic science, 2014, 52.9: 1121-1126 | ca_CA |
dc.rights | © The Author [2013]. Published by Oxford University Press. All rights reserved. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Capillary electrophoresis | ca_CA |
dc.title | Xanthine derivatives quantification in serum by capillary zone electrophoresis | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1093/chromsci/bmt164 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://chromsci.oxfordjournals.org/content/52/9/1121 | ca_CA |
dc.edition | © The Author [2013]. Published by Oxford University Press. All rights reserved. | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
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