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dc.contributor.authorVives-Peris, Vicente
dc.contributor.authorRambla Alegre, María
dc.contributor.authorDurgbanshi, Abhilasha
dc.contributor.authorBose, Devasish
dc.contributor.authorEsteve-Romero, Josep
dc.contributor.authorMarco Peiró, Sergio
dc.date.accessioned2015-06-22T07:18:15Z
dc.date.available2015-06-22T07:18:15Z
dc.date.issued2015-06-22
dc.identifier.issn0021-9665
dc.identifier.otherPERIS-VICENTE, Juan, et al. Xanthine Derivatives Quantification in Serum by Capillary Zone Electrophoresis. Journal of chromatographic science, 2014, 52.9: 1121-1126.
dc.identifier.urihttp://hdl.handle.net/10234/124543
dc.description.abstractA capillary electrophoresis method was developed to quantify caffeine and theophylline, xanthine derivatives with bronchodilator activity. Buffer concentration, pH and applied voltage were optimized using a central composite design-face centred. Separation conditions were: silica capillary tube, 75 μm (i.d.) and 61 cm (total length); absorbance detection, 280 nm; borate buffer, 20 mM, pH 9.0; applied voltage, 25 kV and 1 psi injection/8 s. Validation was performed in blank serum following the International Conference Harmonization guidelines: resolution (peaks without overlapping), linear range (0.125–50 µg/mL; r2 > 0.9999), limits of detection and quantification (10; 20 and 33; 66 ppb for caffeine and theophylline, respectively), intra- and inter-day precision (Relative standard deviation lower than 1.9%) and accuracy (98–101%). Migration times were <8 min. This method is simple, specific and suitable and reaches high label claims (98.7–100.4%) in pharmaceutical formulations analysis. Moreover, the method was applied to the monitoring of the analytes in serum of patients.ca_CA
dc.format.extent16 p.ca_CA
dc.language.isoengca_CA
dc.relation.isPartOfJournal of chromatographic science, 2014, 52.9: 1121-1126ca_CA
dc.rights© The Author [2013]. Published by Oxford University Press. All rights reserved.ca_CA
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/*
dc.subjectCapillary electrophoresisca_CA
dc.titleXanthine derivatives quantification in serum by capillary zone electrophoresisca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1093/chromsci/bmt164
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_CA
dc.relation.publisherVersionhttp://chromsci.oxfordjournals.org/content/52/9/1121ca_CA
dc.edition© The Author [2013]. Published by Oxford University Press. All rights reserved.ca_CA
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_CA


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