A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
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Impacto
Scholar |
Otros documentos de la autoría: Cuenca Ortolá, Irene; Martínez Rojas, Beatriz; Moreno Manzano, Victoria; García Castelló, Marcos; Monleón Pradas, Manuel; Martínez Ramos, Cristina; Más-Estellés, Jorge
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/36080
comunitat-uji-handle3:10234/36082
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INVESTIGACIONMetadatos
Título
A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun MembranesAutoría
Fecha de publicación
2022-11Editor
MDPIISSN
2227-9059Cita bibliográfica
Cuenca-Ortolá, I.; Martínez-Rojas, B.; Moreno-Manzano, V.; García Castelló, M.; Monleón Pradas, M.; Martínez-Ramos, C.; Más Estellés, J. A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes. Biomedicines 2022, 10, 2736. https://doi.org/10.3390/biomedicines10112736Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/2227-9059/10/11/2736Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC ... [+]
Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury. [-]
Publicado en
Biomedicines, 2022, vol. 10, no 11Entidad financiadora
Ministerio de Ciencia, Innovación y Universidades | European Commission | Generalitat Valenciana
Identificador de la entidad financiadora
http://dx.doi.org/10.13039/501100011033
Código del proyecto o subvención
MICIU/ICTI2017-2020/RTI2018-095872-B-C21 | MICIU/ICTI2017-2020/RTI2018-095872-B-C22 | eu-repo/grantAgreement/EC/H2020/964562 | PRE2019-090716 | ACIF/2019/120
Título del proyecto o subvención
Nuevo biomaterial bio-activo para la regeneración de lesiones medulares | Nuevo dispositivo bioactivo para la regeneración de lesiones de la medula espinal
Derechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- MED_Articles [667]