A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
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Other documents of the author: Cuenca Ortolá, Irene; Martínez Rojas, Beatriz; Moreno Manzano, Victoria; García Castelló, Marcos; Monleón Pradas, Manuel; Martínez Ramos, Cristina; Más-Estellés, Jorge
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Title
A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun MembranesAuthor (s)
Date
2022-11Publisher
MDPIISSN
2227-9059Bibliographic citation
Cuenca-Ortolá, I.; Martínez-Rojas, B.; Moreno-Manzano, V.; García Castelló, M.; Monleón Pradas, M.; Martínez-Ramos, C.; Más Estellés, J. A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes. Biomedicines 2022, 10, 2736. https://doi.org/10.3390/biomedicines10112736Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2227-9059/10/11/2736Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC ... [+]
Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury. [-]
Is part of
Biomedicines, 2022, vol. 10, no 11Funder Name
Ministerio de Ciencia, Innovación y Universidades | European Commission | Generalitat Valenciana
Funder ID
http://dx.doi.org/10.13039/501100011033
Project code
MICIU/ICTI2017-2020/RTI2018-095872-B-C21 | MICIU/ICTI2017-2020/RTI2018-095872-B-C22 | eu-repo/grantAgreement/EC/H2020/964562 | PRE2019-090716 | ACIF/2019/120
Project title or grant
Nuevo biomaterial bio-activo para la regeneración de lesiones medulares | Nuevo dispositivo bioactivo para la regeneración de lesiones de la medula espinal
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info:eu-repo/semantics/openAccess
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- MED_Articles [667]