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A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes
dc.contributor.author | Cuenca Ortolá, Irene | |
dc.contributor.author | Martínez Rojas, Beatriz | |
dc.contributor.author | Moreno Manzano, Victoria | |
dc.contributor.author | García Castelló, Marcos | |
dc.contributor.author | Monleón Pradas, Manuel | |
dc.contributor.author | Martínez Ramos, Cristina | |
dc.contributor.author | Más-Estellés, Jorge | |
dc.date.accessioned | 2023-05-08T09:52:23Z | |
dc.date.available | 2023-05-08T09:52:23Z | |
dc.date.issued | 2022-11 | |
dc.identifier.citation | Cuenca-Ortolá, I.; Martínez-Rojas, B.; Moreno-Manzano, V.; García Castelló, M.; Monleón Pradas, M.; Martínez-Ramos, C.; Más Estellés, J. A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes. Biomedicines 2022, 10, 2736. https://doi.org/10.3390/biomedicines10112736 | ca_CA |
dc.identifier.issn | 2227-9059 | |
dc.identifier.uri | http://hdl.handle.net/10234/202415 | |
dc.description.abstract | Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury. | ca_CA |
dc.format.extent | 19 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | MDPI | ca_CA |
dc.relation | Nuevo biomaterial bio-activo para la regeneración de lesiones medulares | ca_CA |
dc.relation | Nuevo dispositivo bioactivo para la regeneración de lesiones de la medula espinal | ca_CA |
dc.relation.isPartOf | Biomedicines, 2022, vol. 10, no 11 | ca_CA |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | ca_CA |
dc.subject | polylactic acid | ca_CA |
dc.subject | aligned substrates | ca_CA |
dc.subject | neural progenitor cells | ca_CA |
dc.subject | magnetic stimulation | ca_CA |
dc.subject | electric stimulation | ca_CA |
dc.title | A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.3390/biomedicines10112736 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://www.mdpi.com/2227-9059/10/11/2736 | ca_CA |
dc.description.sponsorship | Grants RTI2018-095872-B-C21/ERDF and RTI2018-095872-B-C22/ERDF funded by MCIN/AEI//10.13039/501100011033 and by ERDF A way of making Europe. This project was also funded by Project 964562 H2020 FetOpen program. Irene Cuenca Ortolá acknowledges the grant PRE2019-090716 funded by MCIN/AEI/ 10.13039/501100011033 and by ESF Investing in your future. B. Martínez Rojas was supported by a grant from the Consellería de Educación, Investigación, Cultura y Deporte de la Generalitat Valenciana and the European Social Fundation ACIF/2019/120. | |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.identifier | http://dx.doi.org/10.13039/501100011033 | ca_CA |
project.funder.name | Ministerio de Ciencia, Innovación y Universidades | ca_CA |
project.funder.name | European Commission | ca_CA |
project.funder.name | Generalitat Valenciana | ca_CA |
oaire.awardNumber | MICIU/ICTI2017-2020/RTI2018-095872-B-C21 | ca_CA |
oaire.awardNumber | MICIU/ICTI2017-2020/RTI2018-095872-B-C22 | ca_CA |
oaire.awardNumber | eu-repo/grantAgreement/EC/H2020/964562 | ca_CA |
oaire.awardNumber | PRE2019-090716 | ca_CA |
oaire.awardNumber | ACIF/2019/120 | ca_CA |
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