The Cluster Transfer Function of AtNEET Supports the Ferredoxin–Thioredoxin Network of Plant Cells
Impacto
Scholar |
Otros documentos de la autoría: I Zandalinas, Sara; Song, Luhua; Nechushtai, Rachel; Mendoza-Cozatl, David G.; Mittler, Ron
Metadatos
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INVESTIGACIONMetadatos
Título
The Cluster Transfer Function of AtNEET Supports the Ferredoxin–Thioredoxin Network of Plant CellsFecha de publicación
2022-08Editor
MDPIISSN
2076-3921Cita bibliográfica
Zandalinas, S.I.; Song, L.; Nechushtai, R.; Mendoza-Cozatl, D.G.; Mittler, R. The Cluster Transfer Function of AtNEET Supports the Ferredoxin–Thioredoxin Network of Plant Cells. Antioxidants 2022, 11, 1533. https://doi.org/10.3390/antiox11081533Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/2076-3921/11/8/1533Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
arabidopsis | chloroplast | inducible expression | iron–sulfur | NEET | proteomics | ROS | thioredoxin
Resumen
NEET proteins are conserved 2Fe-2S proteins that regulate the levels of iron and reactive oxygen species in plant and mammalian cells. Previous studies of seedlings with constitutive expression of AtNEET, or its ... [+]
NEET proteins are conserved 2Fe-2S proteins that regulate the levels of iron and reactive oxygen species in plant and mammalian cells. Previous studies of seedlings with constitutive expression of AtNEET, or its dominant-negative variant H89C (impaired in 2Fe-2S cluster transfer), revealed that disrupting AtNEET function causes oxidative stress, chloroplast iron overload, activation of iron-deficiency responses, and cell death. Because disrupting AtNEET function is deleterious to plants, we developed an inducible expression system to study AtNEET function in mature plants using a time-course proteomics approach. Here, we report that the suppression of AtNEET cluster transfer function results in drastic changes in the expression of different members of the ferredoxin (Fd), Fd-thioredoxin (TRX) reductase (FTR), and TRX network of Arabidopsis, as well as in cytosolic cluster assembly proteins. In addition, the expression of Yellow Stripe-Like 6 (YSL6), involved in iron export from chloroplasts was elevated. Taken together, our findings reveal new roles for AtNEET in supporting the Fd-TFR-TRX network of plants, iron mobilization from the chloroplast, and cytosolic 2Fe-2S cluster assembly. In addition, we show that the AtNEET function is linked to the expression of glutathione peroxidases (GPXs), which play a key role in the regulation of ferroptosis and redox balance in different organisms. [-]
Publicado en
Antioxidants, 2022, vol. 11, no 8Entidad financiadora
National Science Foundation
Código del proyecto o subvención
IOS-2110017 | IOS-1353886 | MCB-1936590 | IOS-1932639 | MCB-2224839
Derechos de acceso
info:eu-repo/semantics/openAccess
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