Rapid and sensitive determination of nicotine in formulations and biological fluid using micellar liquid chromatography with electrochemical detection
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Show full item recordcomunitat-uji-handle:10234/9
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http://dx.doi.org/10.1016/j.jchromb.2010.07.010 |
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Title
Rapid and sensitive determination of nicotine in formulations and biological fluid using micellar liquid chromatography with electrochemical detectionAuthor (s)
Date
2010Publisher
ElsevierISSN
15700232Bibliographic citation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 878, 26, p. 2397-2402Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/publishedVersionAbstract
Nicotine can be determined in pharmaceuticals and biological fluids by micellar liquid chromatography (MLC) using a C18 column, a mobile phase containing sodium dodecyl sulphate 0.15M-6% (v/v) pentanol-NaH<sub>2</su ... [+]
Nicotine can be determined in pharmaceuticals and biological fluids by micellar liquid chromatography (MLC) using a C18 column, a mobile phase containing sodium dodecyl sulphate 0.15M-6% (v/v) pentanol-NaH<sub>2</sub>PO<sub>4</sub> 0.01M (pH 6)-KCl 0.001M, with electrochemical detection at 0.8V. In the optimization step, the influence of the modifiers propanol, butanol and pentanol, and the voltage has been studied. With the proposed method the analysis time is below than 8min, linearity better than 0.999, limits of detection and quantification (ng/ml) was 4 and 12 respectively, repeatability and intermediate precision below 1.8%, and all these parameters are adequate for the quantification of nicotine in chewing gum, dermal patches, tobacco and serum samples either by a pharmacologist, pathologist or toxicologist. © 2010 Elsevier B.V. [-]
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