A novel in vitro tissue culture approach to study salt stress responses in citrus
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Otros documentos de la autoría: Montoliu Vidal, Almudena; López Climent, María Fernanda; Arbona, Vicent; Perez-Clemente, Rosa Maria; Gomez-Cadenas, Aurelio
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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Título
A novel in vitro tissue culture approach to study salt stress responses in citrusAutoría
Fecha de publicación
2009Editor
Springer VerlagISSN
0167-6903Cita bibliográfica
MONTOLIU, Almudena, et al. A novel in vitro tissue culture approach to study salt stress responses in citrus. Plant Growth Regulation, 2009, vol. 59, no 2, p. 179-187.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://link.springer.com/article/10.1007/s10725-009-9401-0Palabras clave / Materias
Resumen
In citrus, a major crop throughout the world, growth and yield are seriously affected by salinity. Different approaches, including agronomical, physiological and molecular methods, have been used to address this ... [+]
In citrus, a major crop throughout the world, growth and yield are seriously affected by salinity. Different approaches, including agronomical, physiological and molecular methods, have been used to address this problem. In this work, an in vitro experimental system has been developed to study the toxic effect of NaCl on three citrus genotypes, avoiding the ion filter that represents the root system. To carry out the experiments, shoots were obtained from nodal segments of Cleopatra mandarin, Carrizo citrange and citrumelo CPB4475 plants growing in a greenhouse. Shoots were cultured in control or NaCl-supplemented media. After testing several salt concentrations, 60 mM NaCl was selected as saline treatment. Shoots accumulated similar levels of chloride when cultured without roots and exhibited similar leaf damage. No increases in malondialdehyde levels were observed in any genotype (as a measure of oxidative stress). Similar patterns of hormonal signalling (in terms of abscisic acid and salicylic acid contents) were exhibited in the three genotypes, despite their different tolerance under field conditions. All data together indicate that, without root system, all genotypes had the same behaviour under salt stress. The in vitro culture system has been proved as a useful tool to study biochemical processes involved in the response of citrus to salt stress [-]
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Plant Growth Regulation, 2009, v. 52, n. 2, p. 179-187Derechos de acceso
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