A novel in vitro tissue culture approach to study salt stress responses in citrus
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Other documents of the author: Montoliu Vidal, Almudena; López Climent, María Fernanda; Arbona, Vicent; Perez-Clemente, Rosa Maria; Gomez-Cadenas, Aurelio
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comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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Title
A novel in vitro tissue culture approach to study salt stress responses in citrusAuthor (s)
Date
2009Publisher
Springer VerlagISSN
0167-6903Bibliographic citation
MONTOLIU, Almudena, et al. A novel in vitro tissue culture approach to study salt stress responses in citrus. Plant Growth Regulation, 2009, vol. 59, no 2, p. 179-187.Type
info:eu-repo/semantics/articlePublisher version
https://link.springer.com/article/10.1007/s10725-009-9401-0Subject
Abstract
In citrus, a major crop throughout the world, growth and yield are seriously affected by salinity. Different approaches, including agronomical, physiological and molecular methods, have been used to address this ... [+]
In citrus, a major crop throughout the world, growth and yield are seriously affected by salinity. Different approaches, including agronomical, physiological and molecular methods, have been used to address this problem. In this work, an in vitro experimental system has been developed to study the toxic effect of NaCl on three citrus genotypes, avoiding the ion filter that represents the root system. To carry out the experiments, shoots were obtained from nodal segments of Cleopatra mandarin, Carrizo citrange and citrumelo CPB4475 plants growing in a greenhouse. Shoots were cultured in control or NaCl-supplemented media. After testing several salt concentrations, 60 mM NaCl was selected as saline treatment. Shoots accumulated similar levels of chloride when cultured without roots and exhibited similar leaf damage. No increases in malondialdehyde levels were observed in any genotype (as a measure of oxidative stress). Similar patterns of hormonal signalling (in terms of abscisic acid and salicylic acid contents) were exhibited in the three genotypes, despite their different tolerance under field conditions. All data together indicate that, without root system, all genotypes had the same behaviour under salt stress. The in vitro culture system has been proved as a useful tool to study biochemical processes involved in the response of citrus to salt stress [-]
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Plant Growth Regulation, 2009, v. 52, n. 2, p. 179-187Rights
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