dc.contributor.author | Navarro Sánchez, Mónica | |
dc.contributor.author | Gil-Miravet, Isis | |
dc.contributor.author | Montero Caballero, Daniel | |
dc.contributor.author | Bathgate, Ross A. D. | |
dc.contributor.author | Hossain, Mohammed Akhter | |
dc.contributor.author | Castillo-Gómez, Esther | |
dc.contributor.author | Gundlach, Andrew Lawrence | |
dc.contributor.author | Olucha-Bordonau, Francisco E | |
dc.date.accessioned | 2024-06-14T06:17:52Z | |
dc.date.available | 2024-06-14T06:17:52Z | |
dc.date.issued | 2024-07 | |
dc.identifier.citation | M. Navarro-Sánchez, I. Gil-Miravet, D. Montero-Caballero, R. A.D. Bathgate, M. A. Hossain, E. Castillo-Gómez, A. L. Gundlach, F. E. Olucha-Bordonau, Modulation of contextual fear acquisition and extinction by acute and chronic relaxin-3 receptor (RXFP3) activation in the rat retrosplenial cortex, Biochem. Pharmacol. 225 (2024) 116264, https://doi.org/10.1016/j.bcp.2024.116264 | ca_CA |
dc.identifier.isbn | 0006-2952 | |
dc.identifier.issn | 1873-2968 | |
dc.identifier.uri | http://hdl.handle.net/10234/207816 | |
dc.description.abstract | The retrosplenial cortex (RSC) plays a central role in processing contextual fear conditioning. In addition to corticocortical and thalamocortical projections, the RSC receives subcortical inputs, including a substantial projection from the nucleus incertus in the pontine tegmentum. This GABAergic projection contains the neuropeptide, relaxin-3 (RLN3), which inhibits target neurons via its Gi/o-protein-coupled receptor, RXFP3. To assess this peptidergic system role in contextual fear conditioning, we bilaterally injected the RSC of adult rats with an adeno-associated-virus (AAV), expressing the chimeric RXFP3 agonist R3/I5 or a control AAV, and subjected them to contextual fear conditioning. The R3/I5 injected rats did not display any major differences to control-injected and naïve rats but displayed a significantly delayed extinction. Subsequently, we employed acute bilateral injections of the specific RXFP3 agonist peptide, RXFP3-Analogue 2 (A2), into RSC. While the administration of A2 before each extinction trial had no impact on the extinction process, treatment with A2 before each acquisition trial resulted in delayed extinction. In related anatomical studies, we detected an enrichment of RLN3-immunoreactive nerve fibers in deep layers of the RSC, and a higher level of co-localization of RXFP3 mRNA with vesicular GABA transporter (vGAT) mRNA than with vesicular glutamate transporter-1 (vGLUT1) mRNA across the RSC, consistent with an effect of RLN3/RXFP3 signalling on the intrinsic, inhibitory circuits within the RSC. These findings suggest that contextual conditioning processes in the RSC involve, in part, RLN3 afferent modulation of local inhibitory neurons that provides a stronger memory acquisition which, in turn, retards the extinction process. | ca_CA |
dc.description.sponsorShip | Funding for open access charge: CRUE-Universitat Jaume I | |
dc.format.extent | 15 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation | Desarrollo de sistemas peptidérgicos relacionados con comportamiento social. la interacción relaxin3-oxitocina | ca_CA |
dc.relation.isPartOf | Biochemical Pharmacology, 2024, vol. 225 | ca_CA |
dc.rights | Copyright © 2024 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/). | ca_CA |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | ca_CA |
dc.subject | neuropeptide modulation | ca_CA |
dc.subject | G-protein-coupled receptor (GPCR) | ca_CA |
dc.subject | emotion | ca_CA |
dc.subject | GABA | ca_CA |
dc.subject | glutamate | ca_CA |
dc.subject | excitatory/inhibitory balance | ca_CA |
dc.title | Modulation of contextual fear acquisition and extinction by acute and chronic relaxin-3 receptor (RXFP3) activation in the rat retrosplenial cortex | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1016/j.bcp.2024.116264 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://www.sciencedirect.com/science/article/pii/S0006295224002478 | ca_CA |
dc.description.sponsorship | This research was funded by the UJI predoctoral program PREDOC/2021/19 (MN-S), the UJI postdoctoral program POSDOC/2021/19 (IG-M); Fundación Alicia Koplowitz, Spain, grant number 19I436 (FEO-B, EC-G); the Spanish Ministerio de Ciencia, Innovación y Universidades, grant number RTI2018-095698-B-I00 (FEO-B, IG-M, EC-G); AICO Generalitat Valenciana, grant number AICO/2021/246 (EC-G, FEO-B), the Plan Nacional sobre Drogas of the Spanish Ministerio de Sanidad y Consumo grant number 2020I012 (FEO-B and EC-G) and UJI-B2019-54 (FEO-B). Studies at the Florey Institute were supported by the Victorian Government's Operational Infrastructure Support Program. | |
dc.description.sponsorship | Supported by Red Española de Investigación en Estrés/Spanish Network for Stress Research RED2022-134191-T financed by MCIN/AEI /10.13039/501100011033. We are indebted to the confocal facility and animal facility (SEA) of the Servicio Central de Instrumentación Científica (SCIC) of UJI and Victoria Ibáñez from the Statistics Department UJI. | |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.identifier | http://dx.doi.org/10.13039/501100011033 | ca_CA |
project.funder.name | Ministerio de Ciencia, Innovación y Universidades | ca_CA |
project.funder.name | Ministerio de Ciencia e Innovación | ca_CA |
project.funder.name | Universitat Jaume I | ca_CA |
project.funder.name | Fundación Alicia Koplowitz | ca_CA |
project.funder.name | Generalitat Valenciana | ca_CA |
project.funder.name | Plan Nacional sobre Drogas (Ministerio de Sanidad y Consumo) | ca_CA |
oaire.awardNumber | MICIU/ICTI2017-2020/RTI2018-095698-B-I00 | ca_CA |
oaire.awardNumber | RED2022-134191-T | ca_CA |
oaire.awardNumber | PREDOC/2021/19 | ca_CA |
oaire.awardNumber | POSDOC/2021/19 | ca_CA |
oaire.awardNumber | UJI-B2019-54 | ca_CA |
oaire.awardNumber | 19I436 | ca_CA |
oaire.awardNumber | AICO/2021/246 | ca_CA |
oaire.awardNumber | 2020I012 | ca_CA |