Modulation of contextual fear acquisition and extinction by acute and chronic relaxin-3 receptor (RXFP3) activation in the rat retrosplenial cortex
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Other documents of the author: Navarro Sánchez, Mónica; Gil-Miravet, Isis; Montero Caballero, Daniel; Bathgate, Ross A. D.; Hossain, Mohammed Akhter; Castillo-Gómez, Esther; Gundlach, Andrew Lawrence; Olucha-Bordonau, Francisco E
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comunitat-uji-handle2:10234/36080
comunitat-uji-handle3:10234/36082
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INVESTIGACIONMetadata
Title
Modulation of contextual fear acquisition and extinction by acute and chronic relaxin-3 receptor (RXFP3) activation in the rat retrosplenial cortexAuthor (s)
Date
2024-07Publisher
ElsevierISBN
0006-2952ISSN
1873-2968Bibliographic citation
M. Navarro-Sánchez, I. Gil-Miravet, D. Montero-Caballero, R. A.D. Bathgate, M. A. Hossain, E. Castillo-Gómez, A. L. Gundlach, F. E. Olucha-Bordonau, Modulation of contextual fear acquisition and extinction by acute and chronic relaxin-3 receptor (RXFP3) activation in the rat retrosplenial cortex, Biochem. Pharmacol. 225 (2024) 116264, https://doi.org/10.1016/j.bcp.2024.116264Type
info:eu-repo/semantics/articlePublisher version
https://www.sciencedirect.com/science/article/pii/S0006295224002478Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
The retrosplenial cortex (RSC) plays a central role in processing contextual fear conditioning. In addition to corticocortical and thalamocortical projections, the RSC receives subcortical inputs, including a substantial ... [+]
The retrosplenial cortex (RSC) plays a central role in processing contextual fear conditioning. In addition to corticocortical and thalamocortical projections, the RSC receives subcortical inputs, including a substantial projection from the nucleus incertus in the pontine tegmentum. This GABAergic projection contains the neuropeptide, relaxin-3 (RLN3), which inhibits target neurons via its Gi/o-protein-coupled receptor, RXFP3. To assess this peptidergic system role in contextual fear conditioning, we bilaterally injected the RSC of adult rats with an adeno-associated-virus (AAV), expressing the chimeric RXFP3 agonist R3/I5 or a control AAV, and subjected them to contextual fear conditioning. The R3/I5 injected rats did not display any major differences to control-injected and naïve rats but displayed a significantly delayed extinction. Subsequently, we employed acute bilateral injections of the specific RXFP3 agonist peptide, RXFP3-Analogue 2 (A2), into RSC. While the administration of A2 before each extinction trial had no impact on the extinction process, treatment with A2 before each acquisition trial resulted in delayed extinction. In related anatomical studies, we detected an enrichment of RLN3-immunoreactive nerve fibers in deep layers of the RSC, and a higher level of co-localization of RXFP3 mRNA with vesicular GABA transporter (vGAT) mRNA than with vesicular glutamate transporter-1 (vGLUT1) mRNA across the RSC, consistent with an effect of RLN3/RXFP3 signalling on the intrinsic, inhibitory circuits within the RSC. These findings suggest that contextual conditioning processes in the RSC involve, in part, RLN3 afferent modulation of local inhibitory neurons that provides a stronger memory acquisition which, in turn, retards the extinction process. [-]
Is part of
Biochemical Pharmacology, 2024, vol. 225Funder Name
Ministerio de Ciencia, Innovación y Universidades | Ministerio de Ciencia e Innovación | Universitat Jaume I | Fundación Alicia Koplowitz | Generalitat Valenciana | Plan Nacional sobre Drogas (Ministerio de Sanidad y Consumo)
Funder ID
http://dx.doi.org/10.13039/501100011033
Project code
MICIU/ICTI2017-2020/RTI2018-095698-B-I00 | RED2022-134191-T | PREDOC/2021/19 | POSDOC/2021/19 | UJI-B2019-54 | 19I436 | AICO/2021/246 | 2020I012
Project title or grant
Desarrollo de sistemas peptidérgicos relacionados con comportamiento social. la interacción relaxin3-oxitocina
Rights
info:eu-repo/semantics/openAccess
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- MED_Articles [667]