Mostrar el registro sencillo del ítem
Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration
dc.contributor.author | Martínez Santos, Miriam | |
dc.contributor.author | Ybarra, María | |
dc.contributor.author | Oltra Sanchis, Maria | |
dc.contributor.author | Muriach, Maria | |
dc.contributor.author | Romero, Francisco J | |
dc.contributor.author | Pires, Maria Eduarda | |
dc.contributor.author | Sancho Pelluz, Francisco Javier | |
dc.contributor.author | Barcia, Jorge M. | |
dc.date.accessioned | 2024-03-26T12:51:40Z | |
dc.date.available | 2024-03-26T12:51:40Z | |
dc.date.issued | 2024-01-11 | |
dc.identifier.citation | Martínez-Santos M, Ybarra M, Oltra M, Muriach M, Romero FJ, Pires ME, Sancho-Pelluz J, Barcia JM. Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration. International Journal of Molecular Sciences. 2024; 25(2):934. https://doi.org/10.3390/ijms25020934 | ca_CA |
dc.identifier.issn | 1661-6596 | |
dc.identifier.uri | http://hdl.handle.net/10234/206321 | |
dc.description.abstract | Exosomes or small extracellular vesicles (sEVs) represent a pivotal component in intercellular communication, carrying a diverse array of biomolecules. Several factors can affect sEVs release dynamics, as occurs in hyperglycemia or inflammation. In fact, sEVs release has been associated with the promotion of physio-pathological processes. Among the sEVs cargo, microRNAs play an essential role in cell-to-cell regulation. More concretely, miR-205-5p is related to angiogenesis and cell proliferation. The aim of this study is to understand the specific role of sEVs containing miR-205-5p under high glucose conditions. ARPE-19 cells were cultured with high glucose (HG) for 5 days. sEVs were isolated and characterized. sEVs from ARPE-19 were used for angiogenesis and cell proliferation. HG increased sEVs release but downregulated miR-205-5p cargo expression compared to the control. sEVs from HG-treated ARPE-19 cells promoted tube formation and migration processes. In contrast, miR-205-5p overexpression (by mimic transfection) decreased angiogenesis and cell migration. Our results demonstrate how ARPE-19 cells respond to HG challenge by increasing sEVs with weak miR-205-5p cargo. The absence of this miRNA in sEVs is enough to promote angiogenesis. In contrast, restoring sEVs-miR-205-5p levels decreased it. These findings open new possibilities in sEVs-based therapies containing miR-205-5p against angiogenesis. | ca_CA |
dc.format.extent | 12 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | ca_CA |
dc.relation.isPartOf | International Journal of Molecular Sciences. 2024; 25(2) | ca_CA |
dc.rights | © 2024 by the authors. | ca_CA |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | ca_CA |
dc.subject | extracellular vesicles | ca_CA |
dc.subject | miR-205-5p | ca_CA |
dc.subject | angiogenesis | ca_CA |
dc.subject | retinal pigment epithelium | ca_CA |
dc.subject | migration | ca_CA |
dc.title | Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | 10.3390/ijms25020934 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://www.mdpi.com/1422-0067/25/2/934 | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.name | European Commission | ca_CA |
project.funder.name | Instituto de Salud Carlos III | ca_CA |
project.funder.name | Agencia Estatal de Investigación | ca_CA |
oaire.awardNumber | 101073316 | ca_CA |
oaire.awardNumber | PI21/00083 | ca_CA |
oaire.awardNumber | PID2020-117875GB-10 | ca_CA |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
MED_Articles [667]
Articles de publicacions periòdiques