Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration
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Otros documentos de la autoría: Martínez Santos, Miriam; Ybarra, María; Oltra Sanchis, Maria; Muriach, Maria; Romero, Francisco J; Pires, Maria Eduarda; Sancho Pelluz, Francisco Javier; Barcia, Jorge M.
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INVESTIGACIONMetadatos
Título
Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell MigrationAutoría
Fecha de publicación
2024-01-11Editor
Multidisciplinary Digital Publishing Institute (MDPI)ISSN
1661-6596Cita bibliográfica
Martínez-Santos M, Ybarra M, Oltra M, Muriach M, Romero FJ, Pires ME, Sancho-Pelluz J, Barcia JM. Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration. International Journal of Molecular Sciences. 2024; 25(2):934. https://doi.org/10.3390/ijms25020934Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/1422-0067/25/2/934Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Exosomes or small extracellular vesicles (sEVs) represent a pivotal component in intercellular communication, carrying a diverse array of biomolecules. Several factors can affect sEVs release dynamics, as occurs in ... [+]
Exosomes or small extracellular vesicles (sEVs) represent a pivotal component in intercellular communication, carrying a diverse array of biomolecules. Several factors can affect sEVs release dynamics, as occurs in hyperglycemia or inflammation. In fact, sEVs release has been associated with the promotion of physio-pathological processes. Among the sEVs cargo, microRNAs play an essential role in cell-to-cell regulation. More concretely, miR-205-5p is related to angiogenesis and cell proliferation. The aim of this study is to understand the specific role of sEVs containing miR-205-5p under high glucose conditions. ARPE-19 cells were cultured with high glucose (HG) for 5 days. sEVs were isolated and characterized. sEVs from ARPE-19 were used for angiogenesis and cell proliferation. HG increased sEVs release but downregulated miR-205-5p cargo expression compared to the control. sEVs from HG-treated ARPE-19 cells promoted tube formation and migration processes. In contrast, miR-205-5p overexpression (by mimic transfection) decreased angiogenesis and cell migration. Our results demonstrate how ARPE-19 cells respond to HG challenge by increasing sEVs with weak miR-205-5p cargo. The absence of this miRNA in sEVs is enough to promote angiogenesis. In contrast, restoring sEVs-miR-205-5p levels decreased it. These findings open new possibilities in sEVs-based therapies containing miR-205-5p against angiogenesis. [-]
Publicado en
International Journal of Molecular Sciences. 2024; 25(2)Entidad financiadora
European Commission | Instituto de Salud Carlos III | Agencia Estatal de Investigación
Código del proyecto o subvención
101073316 | PI21/00083 | PID2020-117875GB-10
Derechos de acceso
© 2024 by the authors.
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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