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dc.contributor.authorGonzález Magaña, Amaia
dc.contributor.authorAltuna, Jon
dc.contributor.authorQueralt-Martín, María
dc.contributor.authorLargo, Eneko
dc.contributor.authorVelázquez, Carmen
dc.contributor.authorMontánchez, Itxaso
dc.contributor.authorBernal, Patricia
dc.contributor.authorAlcaraz, Antonio
dc.contributor.authorAlbesa-Jové, David
dc.date.accessioned2023-04-19T09:34:10Z
dc.date.available2023-04-19T09:34:10Z
dc.date.issued2022
dc.identifier.citationGonzález-Magaña, A., Altuna, J., Queralt-Martín, M. et al. The P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria. Commun Biol 5, 1189 (2022). https://doi.org/10.1038/s42003-022-04140-yca_CA
dc.identifier.issn2399-3642
dc.identifier.urihttp://hdl.handle.net/10234/202202
dc.description.abstractThe type VI secretion system (T6SS) of Pseudomonas aeruginosa injects effector proteins into neighbouring competitors and host cells, providing a fitness advantage that allows this opportunistic nosocomial pathogen to persist and prevail during the onset of infections. However, despite the high clinical relevance of P. aeruginosa, the identity and mode of action of most P. aeruginosa T6SS-dependent effectors remain to be discovered. Here, we report the molecular mechanism of Tse5-CT, the toxic auto-proteolytic product of the P. aeruginosa T6SS exported effector Tse5. Our results demonstrate that Tse5-CT is a pore-forming toxin that can transport ions across the membrane, causing membrane depolarisation and bacterial death. The membrane potential regulates a wide range of essential cellular functions; therefore, membrane depolarisation is an efficient strategy to compete with other microorganisms in polymicrobial environments.ca_CA
dc.format.extent15 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherNature Researchca_CA
dc.relationEstudio biofísico de los mecanismos de permeabilización de membranas inducidos por canales iónicosca_CA
dc.relation.isPartOfCommunications Biology, 2022, vol. 5, no 1ca_CA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/ca_CA
dc.subjectVI secretion systemca_CA
dc.subjectcrystal structureca_CA
dc.subjectstructural insightsca_CA
dc.subjectnucleotide sequenceca_CA
dc.subjectRHS proteinsca_CA
dc.subjectcolicin-Bca_CA
dc.titleThe P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteriaca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttps://doi.org/10.1038/s42003-022-04140-y
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_CA
dc.relation.publisherVersionhttps://www.nature.com/articles/s42003-022-04140-yca_CA
dc.description.sponsorshipWe gratefully acknowledge the Laboratories of Dr. Daniel Ladant (Institut Pasteur, Paris) and Dr. Victor de Lorenzo (Centro Nacional de Biotecnología, Madrid) for the plasmids received (pKTop and pSEVA plasmids, respectively). Also, we would like to acknowledge the Laboratory of Dr. Joseph Mougous for the P. aeruginosa strains received. The technical assistance from Cristina Civantos and Adrian Ruiz is also very much appreciated. We acknowledge the FGCZ for the mass spectrometry analyses and the technical support (Functional Genomics Center Zurich (FGCZ), University/ETH Zurich). D.A.-J. acknowledges support by the MINECO Contracts CTQ2016-76941-R and PID2021-127816NB-I00, Fundación Biofísica Bizkaia, the Basque Excellence Research Centre (BERC) programme, and IT709-13 and IT1745-22 of the Basque Government, and Fundación BBVA. A.G.-M. acknowledges the financial support received from the Spanish Ministry of Universities and the Grants for the requalification of the Spanish university system for 2021–2023, financed by the European Union-Next Generation EU-Margarita Salas Modality. A.A. acknowledges support from the Spanish Ministry of Science and Innovation (Project 2019-108434GB-I00 funded by MCIN/AEI/10.13039/501100011033), Generalitat Valenciana (project AICO/2020/066) and Universitat Jaume I (project UJI-B2018-53). M.Q.-M. acknowledges support from the Spanish Ministry of Science and Innovation (Project IJC2018-035283-I funded by MCIN/AEI/10.13039/501100011033) and Universitat Jaume I (project UJI-A2020–21). P.B acknowledges the financial support received from the Spanish Ministry of Science and Innovation through the Ramón y Cajal Programme (contract RYC2019-026551-I).
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_CA
project.funder.identifierhttp://dx.doi.org/10.13039/501100003329ca_CA
project.funder.identifierhttp://dx.doi.org/10.13039/501100004837
project.funder.identifierhttp://dx.doi.org/10.13039/501100011033
project.funder.nameMinisterio de Economía y Competitividad (España)ca_CA
project.funder.nameMinisterio de Ciencia e Innovaciónca_CA
project.funder.nameGobierno Vascoca_CA
project.funder.nameMinisterio de Ciencia, Innovación y Universidadesca_CA
project.funder.nameGeneralitat Valencianaca_CA
project.funder.nameUniversitat Jaume Ica_CA
oaire.awardNumberMINECO/CTQ2016-76941-Rca_CA
oaire.awardNumberMICINN/PID2021-127816NB-I00ca_CA
oaire.awardNumberIT709-13ca_CA
oaire.awardNumberIT1745-22ca_CA
oaire.awardNumberMICIU/ICTI2017-2020/PID2021-127816NB-I00ca_CA
oaire.awardNumberAICO/2020/066ca_CA
oaire.awardNumberUJI-B2018-53ca_CA
oaire.awardNumberMICIU/IJC2018-035283-Ica_CA
oaire.awardNumberUJI-A2020–21ca_CA
oaire.awardNumberMICINN/RYC2019-026551-Ica_CA


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