New insights into the search of meat quality biomarkers assisted by Orbitrap Tribrid untargeted metabolite analysis and chemometrics
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Otros documentos de la autoría: Garlito Molina, Borja; Sentandreu, M.A.; Yusà, Vicent; Oliván, Mamen; Pardo Marín, Olga; Sentandreu, Enrique
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Título
New insights into the search of meat quality biomarkers assisted by Orbitrap Tribrid untargeted metabolite analysis and chemometricsAutoría
Fecha de publicación
2023Editor
ElsevierISSN
0308-8146Cita bibliográfica
GARLITO, Borja, et al. New insights into the search of meat quality biomarkers assisted by Orbitrap Tribrid untargeted metabolite analysis and chemometrics. Food chemistry, 2023, 407: 135173.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Metabolite profiles of normal and defective dry, firm and dark (DFD) meat extracts with known ultimate pH
(pHu) values were determined by Orbitrap Tribrid ID-X untargeted analysis coupled to chemometrics. An
intel ... [+]
Metabolite profiles of normal and defective dry, firm and dark (DFD) meat extracts with known ultimate pH
(pHu) values were determined by Orbitrap Tribrid ID-X untargeted analysis coupled to chemometrics. An
intelligent MS3 AcquireXTM workflow firstly approached the unambiguous characterization of detected features
that were subsequently quantified by a complementary MS1 study of biological replicates. Chemometric research
revealed how threonylphenylalanine (overexpressed in normal meats) together to tetradecadienoyl- and
hydroxydodecanoyl-carnitines (both overexpressed in DFD meats) appropriately grouped meat groups assayed.
Robustness of such biomarkers was confirmed through a time-delayed study of a blind set of samples (unknown
pHu) and evidenced limitations of pHu as an isolated parameter for accurate meat quality differentiation. Other
acyl-carnitines also characterized DFD samples, suggesting interferences induced by pre-slaughter stress (PSS) on
lipid catabolism that would explain accumulation of such intermediate metabolites. Results achieved can ease
understanding of biochemical mechanisms underlying meat quality defects. [-]
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Food chemistry, 2023, 407: 135173Entidad financiadora
Spanish Ministerio de Universidades | Spanish Agencia Estatal de Investigación
Código del proyecto o subvención
MGS/2021/25 (UP2021-021) | RTI2018-096162-R-C22
Derechos de acceso
info:eu-repo/semantics/openAccess
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