New insights into the search of meat quality biomarkers assisted by Orbitrap Tribrid untargeted metabolite analysis and chemometrics
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Other documents of the author: Garlito Molina, Borja; Sentandreu, M.A.; Yusà, Vicent; Oliván, Mamen; Pardo Marín, Olga; Sentandreu, Enrique
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comunitat-uji-handle2:10234/33596
comunitat-uji-handle3:10234/33597
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Title
New insights into the search of meat quality biomarkers assisted by Orbitrap Tribrid untargeted metabolite analysis and chemometricsAuthor (s)
Date
2023Publisher
ElsevierISSN
0308-8146Bibliographic citation
GARLITO, Borja, et al. New insights into the search of meat quality biomarkers assisted by Orbitrap Tribrid untargeted metabolite analysis and chemometrics. Food chemistry, 2023, 407: 135173.Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Metabolite profiles of normal and defective dry, firm and dark (DFD) meat extracts with known ultimate pH
(pHu) values were determined by Orbitrap Tribrid ID-X untargeted analysis coupled to chemometrics. An
intel ... [+]
Metabolite profiles of normal and defective dry, firm and dark (DFD) meat extracts with known ultimate pH
(pHu) values were determined by Orbitrap Tribrid ID-X untargeted analysis coupled to chemometrics. An
intelligent MS3 AcquireXTM workflow firstly approached the unambiguous characterization of detected features
that were subsequently quantified by a complementary MS1 study of biological replicates. Chemometric research
revealed how threonylphenylalanine (overexpressed in normal meats) together to tetradecadienoyl- and
hydroxydodecanoyl-carnitines (both overexpressed in DFD meats) appropriately grouped meat groups assayed.
Robustness of such biomarkers was confirmed through a time-delayed study of a blind set of samples (unknown
pHu) and evidenced limitations of pHu as an isolated parameter for accurate meat quality differentiation. Other
acyl-carnitines also characterized DFD samples, suggesting interferences induced by pre-slaughter stress (PSS) on
lipid catabolism that would explain accumulation of such intermediate metabolites. Results achieved can ease
understanding of biochemical mechanisms underlying meat quality defects. [-]
Is part of
Food chemistry, 2023, 407: 135173Funder Name
Spanish Ministerio de Universidades | Spanish Agencia Estatal de Investigación
Project code
MGS/2021/25 (UP2021-021) | RTI2018-096162-R-C22
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info:eu-repo/semantics/openAccess
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