Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells
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Otros documentos de la autoría: Bessa, Maria João; Brandão, Fátima; Fokkens, Paul H. B.; Leseman, Daan L. A. C.; Boere, A. John F.; Cassee, Flemming R.; Salmatonidis, Apostolos; Viana, Mar; Monfort, Eliseo; Fraga, Sónia; Paulo Teixeira, Joao
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comunitat-uji-handle2:10234/7033
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Título
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial CellsAutoría
Fecha de publicación
2022-04-13Editor
MDPIISSN
1422-0067Cita bibliográfica
Bessa, M.J.; Brandão, F.; Fokkens, P.H.B.; Leseman, D.L.A.C.; Boere, A.J.F.; Cassee, F.R.; Salmatonidis, A.; Viana, M.; Monfort, E.; Fraga, S.; et al. Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells. Int. J. Mol. Sci. 2022, 23, 4278. https://doi.org/10.3390/ ijms23084278Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
High-energy industrial processes have been associated with particle release into workplace
air that can adversely affect workers’ health. The present study assessed the toxicity of incidental
fine (PGFP) and nanop ... [+]
High-energy industrial processes have been associated with particle release into workplace
air that can adversely affect workers’ health. The present study assessed the toxicity of incidental
fine (PGFP) and nanoparticles (PGNP) emitted from atmospheric plasma (APS) and high-velocity
oxy-fuel (HVOF) thermal spraying. Lactate dehydrogenase (LDH) release, 2-(4-nitrophenyl)-2H5-tetrazolio]-1,3-benzene disulfonate (WST-1) metabolisation, intracellular reactive oxygen species
(ROS) levels, cell cycle changes, histone H2AX phosphorylation (γ-H2AX) and DNA damage were
evaluated in human alveolar epithelial cells at 24 h after exposure. Overall, HVOF particles were
the most cytotoxic to human alveolar cells, with cell viability half-maximal inhibitory concentration
(IC50) values of 20.18 µg/cm2 and 1.79 µg/cm2
for PGFP and PGNP, respectively. Only the highest
tested concentration of APS-PGFP caused a slight decrease in cell viability. Particle uptake, cell cycle
arrest at S + G2/M and γ-H2AX augmentation were observed after exposure to all tested particles.
However, higher levels of γ-H2AX were found in cells exposed to APS-derived particles (~16%),
while cells exposed to HVOF particles exhibited increased levels of oxidative damage (~17% tail
intensity) and ROS (~184%). Accordingly, APS and HVOF particles seem to exert their genotoxic
effects by different mechanisms, highlighting that the health risks of these process-generated particles
at industrial settings should not be underestimated. [-]
Publicado en
International Journal of Molecular Sciences. Vol. 23, Issue 8 (April-2 2022)Entidad financiadora
ERA-NET SIINN | Portuguese Foundation for Science and Technology | Operational Program for Competitiveness and Internationalization (POCI) | European Regional Development Funds (FEDER/FNR) | Ministerio de Ciencia e Innovación | University of Porto
Código del proyecto o subvención
FCT; SIINN/0004/2014 | PTDC/MED-TOX/31162/2017 | PCIN-2015-173-C02-01 | CEX2018-000794-S-Severo Ochoa | SFRH/BD/120646/2016 | SFRH/BD/101060/2014 | DL-57/INSA-06/2018 | UIDB/04750/2020 | LA/P/0064/2020
Título del proyecto o subvención
CERASAFE | NanoBioBarriers | PhD scholarships under the framework of the Human Capital Operating Program (POCH)
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