Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responses
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Altres documents de l'autoria: Cerqueira, Andreia; Romero-Gavilán, Francisco J; García-Arnáez, Iñaki; Martinez Ramos, Cristina; Ozturan, Seda; Iloro, Ibon; Azkargorta, Mikel; Elortza, Felix; Izquierdo Escrig, Raul; GURRUCHAGA, MARILO; Goñi, Isabel; Suay, Julio
Metadades
Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
comunitat-uji-handle4:
INVESTIGACIONMetadades
Títol
Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responsesAutoria
Data de publicació
2020-12-30Editor
ElsevierISSN
0928-4931Cita bibliogràfica
CERQUEIRA, A., et al. Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responses. Materials Science and Engineering: C, 2021, vol. 121, p. 111839.Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://www.sciencedirect.com/science/article/pii/S0928493120337589Versió
info:eu-repo/semantics/acceptedVersionParaules clau / Matèries
Resum
Zinc is an essential element with an important role in stimulating the osteogenesis and mineralization and suppressing osteoclast differentiation. In this study, new bioactive ZnCl2-doped sol-gel materials were designed ... [+]
Zinc is an essential element with an important role in stimulating the osteogenesis and mineralization and suppressing osteoclast differentiation. In this study, new bioactive ZnCl2-doped sol-gel materials were designed to be applied as coatings onto titanium. The biomaterials were physicochemically characterized and the cellular responses evaluated in vitro using MC3T3-E1 osteoblasts and RAW264.7 macrophages. The effect of Zn on the adsorption of human serum proteins onto the material surface was evaluated through nLC-MS/MS. The incorporation of Zn did not affect the crosslinking of the sol-gel network. A controlled Zn2+ release was obtained, reaching values below 10 ppm after 21 days. The materials were no cytotoxic and lead to increased gene expression of ALP, TGF-β, and RUNX2 in the osteoblasts. In macrophages, an increase of IL-1β, TGF-β, and IL-4 gene expression was accompanied by a reduced TNF-α liberation. Proteomic results showed changes in the adsorption patterns of proteins associated with immunological, coagulative, and regenerative functions, in a Zn dose-dependent manner. The variations in protein adsorption might lead to the downregulation of the NF-κB pathway, thus explain the observed biological effects of Zn incorporation into biomaterials. Overall, these coatings demonstrated their potential to promote bone tissue regeneration. [-]
Publicat a
Materials Science and Engineering: C, 2021, vol. 121.Entitat finançadora
MINECO | Generalitat Valenciana | Universitat Jaume I | Universidad del País Vasco | Gobierno Vasco
Codi del projecte o subvenció
MAT2017-86043-R; RTC- 2017-6147-1 | GRISOLIAP/2018/091 | UJI-B2017-37 | Posdoc/2019/28 | GIU18/189 | PRE_2017_2_0044
Drets d'accés
0928-4931/© 2020 Elsevier B.V. All rights reserved.
Licencia CC-BY-NC-ND
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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