Mostrar el registro sencillo del ítem
Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responses
dc.contributor.author | Cerqueira, Andreia | |
dc.contributor.author | Romero-Gavilán, Francisco J | |
dc.contributor.author | García-Arnáez, Iñaki | |
dc.contributor.author | Martinez Ramos, Cristina | |
dc.contributor.author | Ozturan, Seda | |
dc.contributor.author | Iloro, Ibon | |
dc.contributor.author | Azkargorta, Mikel | |
dc.contributor.author | Elortza, Felix | |
dc.contributor.author | Izquierdo Escrig, Raul | |
dc.contributor.author | GURRUCHAGA, MARILO | |
dc.contributor.author | Goñi, Isabel | |
dc.contributor.author | Suay, Julio | |
dc.date.accessioned | 2021-02-24T12:35:34Z | |
dc.date.available | 2021-02-24T12:35:34Z | |
dc.date.issued | 2020-12-30 | |
dc.identifier.citation | CERQUEIRA, A., et al. Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responses. Materials Science and Engineering: C, 2021, vol. 121, p. 111839. | ca_CA |
dc.identifier.issn | 0928-4931 | |
dc.identifier.uri | http://hdl.handle.net/10234/192255 | |
dc.description.abstract | Zinc is an essential element with an important role in stimulating the osteogenesis and mineralization and suppressing osteoclast differentiation. In this study, new bioactive ZnCl2-doped sol-gel materials were designed to be applied as coatings onto titanium. The biomaterials were physicochemically characterized and the cellular responses evaluated in vitro using MC3T3-E1 osteoblasts and RAW264.7 macrophages. The effect of Zn on the adsorption of human serum proteins onto the material surface was evaluated through nLC-MS/MS. The incorporation of Zn did not affect the crosslinking of the sol-gel network. A controlled Zn2+ release was obtained, reaching values below 10 ppm after 21 days. The materials were no cytotoxic and lead to increased gene expression of ALP, TGF-β, and RUNX2 in the osteoblasts. In macrophages, an increase of IL-1β, TGF-β, and IL-4 gene expression was accompanied by a reduced TNF-α liberation. Proteomic results showed changes in the adsorption patterns of proteins associated with immunological, coagulative, and regenerative functions, in a Zn dose-dependent manner. The variations in protein adsorption might lead to the downregulation of the NF-κB pathway, thus explain the observed biological effects of Zn incorporation into biomaterials. Overall, these coatings demonstrated their potential to promote bone tissue regeneration. | ca_CA |
dc.format.extent | 26 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Materials Science and Engineering: C, 2021, vol. 121. | ca_CA |
dc.rights | 0928-4931/© 2020 Elsevier B.V. All rights reserved. Licencia CC-BY-NC-ND | ca_CA |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Proteomics | ca_CA |
dc.subject | Bioinorganic chemistry | ca_CA |
dc.subject | Biomaterials | ca_CA |
dc.subject | Bone regeneration | ca_CA |
dc.subject | Hybrids | ca_CA |
dc.title | Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responses | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1016/j.msec.2020.111839 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://www.sciencedirect.com/science/article/pii/S0928493120337589 | ca_CA |
dc.type.version | info:eu-repo/semantics/acceptedVersion | ca_CA |
project.funder.name | MINECO | ca_CA |
project.funder.name | Generalitat Valenciana | ca_CA |
project.funder.name | Universitat Jaume I | ca_CA |
project.funder.name | Universidad del País Vasco | ca_CA |
project.funder.name | Gobierno Vasco | ca_CA |
oaire.awardNumber | MAT2017-86043-R; RTC- 2017-6147-1 | ca_CA |
oaire.awardNumber | GRISOLIAP/2018/091 | ca_CA |
oaire.awardNumber | UJI-B2017-37 | ca_CA |
oaire.awardNumber | Posdoc/2019/28 | ca_CA |
oaire.awardNumber | GIU18/189 | ca_CA |
oaire.awardNumber | PRE_2017_2_0044 | ca_CA |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
ESID_Articles [478]