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A possible use of melatonin in the dental field: Protein adsorption andinvitrocell response on coated titanium
dc.contributor.author | Cerqueira, Andreia | |
dc.contributor.author | Romero-Gavilán, Francisco J | |
dc.contributor.author | Araújo-Gomes, Nuno | |
dc.contributor.author | García-Arnáez, Iñaki | |
dc.contributor.author | Martinez Ramos, Cristina | |
dc.contributor.author | Ozturan, Seda | |
dc.contributor.author | Azkargorta, Mikel | |
dc.contributor.author | Elortza, Felix | |
dc.contributor.author | GURRUCHAGA, MARILO | |
dc.contributor.author | Suay, Julio | |
dc.contributor.author | Goñi, Isabel | |
dc.date.accessioned | 2020-12-01T12:16:11Z | |
dc.date.available | 2020-12-01T12:16:11Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | CERQUEIRA, Andreia, et al. A possible use of melatonin in the dental field: protein adsorption and in vitro cell response on coated titanium. Materials Science and Engineering: C, 2020, vol. 116, p. 111262. | ca_CA |
dc.identifier.issn | 0928-4931 | |
dc.identifier.uri | http://hdl.handle.net/10234/190631 | |
dc.description.abstract | Melatonin (MLT) is widely known for regulating the circadian cycles and has been studied for its role in boneregeneration and inflammation. Its application as a coating for dental implants can condition the local micro-environment, affecting protein deposition on its surface and the cellular and tissue response. Using sol-gelcoatings as a release vehicle for MLT, the aim of this work was to assess the potential of this molecule inimproving the osseointegration and inflammatory responses of a titanium substrate. The materials obtained werephysicochemically characterized (scanning electron microscopy, contact angle, roughness, Fourier-transforminfrared spectroscopy, nuclear magnetic resonance, Si release, MLT liberation, and degradation) and studiedinvitrowith MC3T3-E1 osteoblastic cells and RAW264.7 macrophage cells. Although MLT application led to anincreased gene expression of RUNX2 and BMP2 in 10MTL, it did not improve ALP activity. On the other hand,MLT-enriched sol-gel materials presented potential effects in the adsorption of proteins related to inflammation,coagulation and angiogenesis pathways depending on the dosage used. Using LC-MS/MS, protein adsorptionpatterns were studied after incubation with human serum. Proteins related to the complement systems (CO7,IC1, CO5, CO8A, and CO9) were less adsorbed in materials with MLT; on the other hand, proteins with functionsin the coagulation and angiogenesis pathways, such as A2GL and PLMN, showed a significant adsorption pattern. | ca_CA |
dc.format.extent | 24 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Materials Science and Engineering: C, Volume 116, 2020. | ca_CA |
dc.rights | 0928-4931/ © 2020 Elsevier B.V. All rights reserved. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Osseointegration | ca_CA |
dc.subject | hybrid sol-gel | ca_CA |
dc.subject | inflammation | ca_CA |
dc.subject | proteomics | ca_CA |
dc.subject | coating | ca_CA |
dc.subject | N-acetyl-5-metoxy- tryptamine | ca_CA |
dc.title | A possible use of melatonin in the dental field: Protein adsorption andinvitrocell response on coated titanium | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1016/j.msec.2020.111262 | |
dc.relation.projectID | MAT MAT2017-86043-R;RTC-2017-6147, JI-B2017-37;POSDOC/2019/28, RISOLIAP/2018/091, UFI11/56, PRE_2017_2_0044, The ProteoRed-ISCIII (Grant PRB3 IPT17/0019), SEV-2016-0644 | ca_CA |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://www.sciencedirect.com/science/article/pii/S0928493120306639 | ca_CA |
dc.type.version | info:eu-repo/semantics/submittedVersion | ca_CA |
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