A possible use of melatonin in the dental field: Protein adsorption andinvitrocell response on coated titanium
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Título
A possible use of melatonin in the dental field: Protein adsorption andinvitrocell response on coated titaniumAutoría
Fecha de publicación
2020Editor
ElsevierISSN
0928-4931Cita bibliográfica
CERQUEIRA, Andreia, et al. A possible use of melatonin in the dental field: protein adsorption and in vitro cell response on coated titanium. Materials Science and Engineering: C, 2020, vol. 116, p. 111262.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.sciencedirect.com/science/article/pii/S0928493120306639Versión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
Melatonin (MLT) is widely known for regulating the circadian cycles and has been studied for its role in boneregeneration and inflammation. Its application as a coating for dental implants can condition the local ... [+]
Melatonin (MLT) is widely known for regulating the circadian cycles and has been studied for its role in boneregeneration and inflammation. Its application as a coating for dental implants can condition the local micro-environment, affecting protein deposition on its surface and the cellular and tissue response. Using sol-gelcoatings as a release vehicle for MLT, the aim of this work was to assess the potential of this molecule inimproving the osseointegration and inflammatory responses of a titanium substrate. The materials obtained werephysicochemically characterized (scanning electron microscopy, contact angle, roughness, Fourier-transforminfrared spectroscopy, nuclear magnetic resonance, Si release, MLT liberation, and degradation) and studiedinvitrowith MC3T3-E1 osteoblastic cells and RAW264.7 macrophage cells. Although MLT application led to anincreased gene expression of RUNX2 and BMP2 in 10MTL, it did not improve ALP activity. On the other hand,MLT-enriched sol-gel materials presented potential effects in the adsorption of proteins related to inflammation,coagulation and angiogenesis pathways depending on the dosage used. Using LC-MS/MS, protein adsorptionpatterns were studied after incubation with human serum. Proteins related to the complement systems (CO7,IC1, CO5, CO8A, and CO9) were less adsorbed in materials with MLT; on the other hand, proteins with functionsin the coagulation and angiogenesis pathways, such as A2GL and PLMN, showed a significant adsorption pattern. [-]
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Materials Science and Engineering: C, Volume 116, 2020.Proyecto de investigación
MAT MAT2017-86043-R;RTC-2017-6147, JI-B2017-37;POSDOC/2019/28, RISOLIAP/2018/091, UFI11/56, PRE_2017_2_0044, The ProteoRed-ISCIII (Grant PRB3 IPT17/0019), SEV-2016-0644Derechos de acceso
0928-4931/ © 2020 Elsevier B.V. All rights reserved.
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info:eu-repo/semantics/openAccess
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