Determination of organic contaminants in water samples by Gas Chromatography coupled to tandem Mass Spectrometry (GC-MS/MS)

Abstract

The main objective of this Degree Final Project is to explore the analytical capabilities of gas chromatography coupled to tandem mass spectrometry (GC MS/MS) using triple quadrupole (QqQ) analyzer for the determination of organophosphorus (OP) insecticides in water samples. The study of their capabilities, limitations and ways of working has allowed us to know the instrumental equipment in order to focus on the determination and quantification of pesticides in surface and underground water. During the completion of this degree final project, the following specific objectives have been completed: 1. Development and validation of analytical methodology based on GC MS/MS (QqQ) for quantification and confirmation of three organic contaminants at ppb concentration levels in water, using solid phase extraction (SPE) as extraction/preconcentration technique. 2. Study and optimization of chromatographic methodology by GC-MS/MS (QqQ) for the determination of chlorfenvinfos, chlorpyrifos-methyl and chlorpyrifos-ethyl, giving special attention to the correct identification and confirmation of detected compounds. 3. Learn to operate the instrument and its software making the optimizations and verifications required to ensure that the conditions of the measurements are correct, including the programming methods and the sample analysis sequences. 4. Study and optimization of SPE using C18 and HLB cartridges for the determination of analytes selected in superficial water samples. 5. Application of the developed procedure to real water samples. 6. Acquire knowledge to be able to interpret the results from chromatograms, tables or other and thus be able to make corrections, optimizations and collection of appropriate conclusions. 7. Finally, know and see the manner of working in a research laboratory, more professional than the academic laboratory of the University.