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Determination of mycotoxins in different food commodities by ultra-high-pressure liquid chromatography coupled to triple quadrupole mass spectrometry
dc.contributor.author | Beltrán Iturat, Eduardo | |
dc.contributor.author | Ibáñez, Maria | |
dc.contributor.author | Sancho, Juan V | |
dc.contributor.author | Hernandez, Felix | |
dc.date.accessioned | 2014-07-08T13:43:06Z | |
dc.date.available | 2014-07-08T13:43:06Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0951-4198 | |
dc.identifier.uri | http://hdl.handle.net/10234/97165 | |
dc.description.abstract | A rapid multianalyte-multiclass method with little sample manipulation has been developed for the simultaneous determination of eleven mycotoxins in different food commodities by using ultra-high-pressure liquid chromatography coupled to triple quadrupole mass spectrometry (UHPLC/MS/MS). Toxins were extracted from the samples with acetonitrile/water (80:20, v/v) 0.1% HCOOH and, after a two-fold dilution with water, directly injected into the system. Thanks to the fast high-resolution separation of UHPLC, the eleven mycotoxins were separated by gradient elution in only 4 min. The method has been validated in three food matrices (maize kernels, dry pasta (wheat), and eight-multicereal babyfood (wheat, maize, rice, oat, barley, rye, sorghum, millet)) at four different concentration levels. Satisfactory recoveries were obtained (70–110%) and precision (expressed as relative standard deviation) was typically below 15% with very few exceptions. Quantification of samples was carried out with matrix-matched standards calibration. The lowest concentration successfully validated in sample was as low as 0.5 µg/kg for aflatoxins and ochratoxin A in babyfood, and 20 µg/kg for the rest of the selected mycotoxins in all matrices tested. Deoxynivalenol could be only validated at 200 µg/kg, due the poor sensitivity for this mycotoxin analysis. With only two exceptions (HT-2 and deoxynivalenol), the limits of detection (LODs), estimated for a signal-to-noise ratio of 3 from the chromatograms of samples spiked at the lowest level validated, varied between 0.1 and 1 µg/kg in the three food matrices tested. The method was applied to the analysis of different kinds of samples. Positive findings were confirmed by acquiring two transitions (Q quantification, q confirmation) and evaluating the Q/q ratio. | ca_CA |
dc.format.extent | 9 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Wiley | ca_CA |
dc.relation.isPartOf | Rapid Communications in Mass Spectrometry, 23, 12, p. 1801–1809 | ca_CA |
dc.rights | Copyright © 2009 John Wiley & Sons, Ltd. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | mycotoxins | ca_CA |
dc.subject | chromatography | ca_CA |
dc.subject | mass spectrometry | ca_CA |
dc.title | Determination of mycotoxins in different food commodities by ultra-high-pressure liquid chromatography coupled to triple quadrupole mass spectrometry | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1002/rcm.4077 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://onlinelibrary.wiley.com/doi/10.1002/rcm.4077/full | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion |
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