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dc.contributor.authorLogan, Angela
dc.contributor.authorCochemé, Helena M.
dc.contributor.authorBoon Li Pun, Pamela
dc.contributor.authorApostolova, Nadezda
dc.contributor.authorSmith, Robin A. J.
dc.contributor.authorLarsen, Lesley
dc.contributor.authorLarsen, David S.
dc.contributor.authorJames, Andrew M.
dc.contributor.authorFearnley, Ian M.
dc.contributor.authorRogatti, Sebastian
dc.contributor.authorPrime, Tracy A.
dc.contributor.authorFinichiu, Peter G.
dc.contributor.authorDare, Anna
dc.contributor.authorChouchani, Edward T.
dc.contributor.authorPell, Victoria R.
dc.contributor.authorMethner, Carmen
dc.contributor.authorQuin, Caroline
dc.contributor.authorMcQuaker, Stephen J.
dc.contributor.authorKrieg, Thomas
dc.contributor.authorHartley, Richard C.
dc.contributor.authorMurphy, Michael P.
dc.date.accessioned2014-05-19T11:55:20Z
dc.date.available2014-05-19T11:55:20Z
dc.date.issued2014-02
dc.identifier.citationLOGAN, Angela, et al. Using exomarkers to assess mitochondrial reactive species< i> in vivo</i>. Biochimica et Biophysica Acta (BBA)-General Subjects, 2014, vol. 1840, no 2, p. 923-930ca_CA
dc.identifier.issn0304-4165
dc.identifier.urihttp://hdl.handle.net/10234/92472
dc.description.abstractBackground The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging. Scope of review One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and amounts of the reactive species present in vivo. This is akin to the measurement of biomarkers produced by the interaction of reactive species with endogenous biomolecules. Major conclusions and general significance Our laboratories have developed mitochondria-targeted probes that generate exomarkers that can be analysed ex vivo by mass spectrometry to assess levels of reactive species within mitochondria in vivo. We have used one of these compounds, MitoB, to infer the levels of mitochondrial hydrogen peroxide within flies and mice. Here we describe the development of MitoB and expand on this example to discuss how better probes and exomarkers can be developed. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.ca_CA
dc.format.extent8 p.ca_CA
dc.format.mimetypeapplication/pdfca_CA
dc.language.isoengca_CA
dc.publisherElsevierca_CA
dc.relation.isPartOfBiochimica et Biophysica Acta (BBA)-General Subjects, 2014, vol. 1840, no 2ca_CA
dc.rightsCopyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.ca_CA
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/*
dc.subjectExomarkerca_CA
dc.subjectMitochondriaca_CA
dc.subjectMitoBca_CA
dc.subjectMass spectrometryca_CA
dc.subjectOxidative damageca_CA
dc.subjectReactive oxygen speciesca_CA
dc.titleUsing exomarkers to assess mitochondrial reactive species in vivoca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbagen.2013.05.026
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_CA
dc.relation.publisherVersionhttp://www.sciencedirect.com/science/article/pii/S0304416513002225#ca_CA
dc.type.versioninfo:eu-repo/semantics/publishedVersion


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