Submicellar and micellar reversed-phase liquid chromatographic modes applied to the separation of β-blockers
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Otros documentos de la autoría: Ruiz Ángel, M. J.; Torres Lapasio, José Ramón; García Álvarez-Coque, M. C.; Carda-Broch, Samuel
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
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http://dx.doi.org/10.1016/j.chroma.2009.02.016 |
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Título
Submicellar and micellar reversed-phase liquid chromatographic modes applied to the separation of β-blockersAutoría
Fecha de publicación
2009Editor
ElsevierISSN
219673Cita bibliográfica
Journal of Chromatography A, 1216, 15, p. 3199-3209Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
The behaviour of a reversed-phase liquid chromatographic (RPLC) system (i.e. elution order, resolution and analysis time), used in the analysis of β-blockers with acetonitrile-water mobile phases, changes drastically ... [+]
The behaviour of a reversed-phase liquid chromatographic (RPLC) system (i.e. elution order, resolution and analysis time), used in the analysis of β-blockers with acetonitrile-water mobile phases, changes drastically upon addition of an anionic surfactant (sodium dodecyl sulphate, SDS). Surfactant monomers cover the alkyl-bonded phase in different extent depending on the concentration of both modifiers, in the ranges 1 × 10<sup>-3</sup>-0.15 M SDS and 5-50% acetonitrile. Meanwhile, the surfactant is dissolved in the mobile phase as free monomers, associated in small clusters or forming micelles. Four characteristic RPLC modes are yielded, with transition regions between them: hydro-organic, micellar, and low and high submicellar. The mobile phases in the two latter modes contain a concentration of SDS below or well above the critical micellar concentration (CMC) in water (i.e. 8 × 10<sup>-3</sup> M), and more than 30% acetonitrile. High submicellar RPLC appeared as the most promising mode, as it allowed full resolution of the β-blockers in practical times, while these were unresolved or highly retained in the other RPLC modes. The strong attraction of the cationic solutes to the anionic SDS makes a direct transfer mechanism between surfactant molecules in the stationary and mobile phases likely. © 2009 Elsevier B.V. All rights reserved. [-]
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