Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene Expression
Ver/ Abrir
Impacto
Scholar |
Otros documentos de la autoría: Salesa Landete, Beatriz; Tuñón Molina, Alberto; Cano-Vicent, Alba; Assis, Marcelo de; Andres, Juan; Serrano-Aroca, Ángel
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene ExpressionAutoría
Fecha de publicación
2022-01-12Editor
MDPIISSN
2076-3417Cita bibliográfica
Salesa, B.; Tuñón-Molina, A.; Cano-Vicent, A.; Assis, M.; Andrés, J.; Serrano-Aroca, Á. Graphene Nanoplatelets: In Vivo and In Vitro Toxicity, Cell Proliferative Activity, and Cell Gene Expression. Appl. Sci. 2022, 12, 720. https:// doi.org/10.3390/app12020720Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Multi-layer graphene (2–10 layers), also called graphene nanoplatelets (GNPs), is a carbonbased nanomaterial (CBN) type with excellent properties desirable for many biomedical applications.
Despite the promising ... [+]
Multi-layer graphene (2–10 layers), also called graphene nanoplatelets (GNPs), is a carbonbased nanomaterial (CBN) type with excellent properties desirable for many biomedical applications.
Despite the promising advantages reported of GNPs, nanoscale materials may also present a potential
hazard to humans. Therefore, in this study, the in vivo toxicity of these nanomaterials at a wide range
of concentrations from 12.5 to 500 µg/mL was evaluated in the Caenorhabditis elegans model for 24 h
(acute toxicity) and 72 h (chronic toxicity). Furthermore, their in vitro toxicity (from 0 to 10 µg/mL
for 12 and 24 h), proliferative activity at 72 and 96 h, and their effect on the expression of thirteen
genes in human keratinocytes HaCaT cells were studied. The physico-chemical and morphological
aspects of the GNPs used in this study were analyzed by Raman scattering spectroscopy, electron
microscopy, zeta potential as a function of pH, and particle size measurements by dynamic light
scattering. The results of this study showed that GNPs showed in vivo non-toxic concentrations of
25 and 12.5 µg/mL for 24 h, and at 12.5 µg/mL for 72 h. Moreover, GNPs present time-dependent
cytotoxicity (EC50 of 1.142 µg/mL and 0.760 µg/mL at 12 h and 24 h, respectively) and significant
proliferative activity at the non-toxic concentrations of 0.005 and 0.01 µg/mL in the HaCaT cell line.
The gene expression study showed that this multi-layer-graphene is capable of up-regulating six of
the thirteen genes of human keratinocytes (SOD1, CAT, TGFB1, FN1, CDH1, and FBN), two more
genes than other CBNs in their oxidized form such as multi-layer graphene oxide. Therefore, all these
results reinforce the promising use of these CBNs in biomedical fields such as wound healing and
skin tissue engineering. [-]
Publicado en
Appl. Sci. 2022, 12, 720Entidad financiadora
Universidad Católica de Valencia San Vicente Mártir, | Ministerio de Ciencia e Innovación | Universitat Jaume I de Castellón | Generalitat Valenciana | Ministerio de Ciencia, Innovación y Universidades (Spain) | Fundação de Amparo à Pesquisa do Estado de São Paulo— FAPESP | Conselho Nacional de Desenvolvimento Cientifico e Tecnológico | CAPES
Código del proyecto o subvención
Grant 2020-231-006UCV | (PID2020- 119333RB-I00/AEI/10.13039/501100011033) | UJI-B2019-30 | AICO2020 | PGC2018- 094417-B-I00 | 2013/07296-2 | 166281/2017-4 | 001
Derechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- QFA_Articles [818]