Putrescine biosynthetic pathways modulate root growth differently in tomato seedlings grown under different N sources
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Otros documentos de la autoría: González-Hernández, Ana I.; Scalschi, Loredana; Troncho, Pilar; García Agustín, Pilar; Camañes, Gemma
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Título
Putrescine biosynthetic pathways modulate root growth differently in tomato seedlings grown under different N sourcesAutoría
Fecha de publicación
2021-11-16Editor
Elsevier; Urban and FischerISSN
0176-1617Cita bibliográfica
GONZÁLEZ-HERNÁNDEZ, Ana Isabel, et al. Putrescine biosynthetic pathways modulate root growth differently in tomato seedlings grown under different N sources. Journal of Plant Physiology, 2022, vol. 268, p. 153560.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
The biosynthesis of putrescine is mainly driven by arginine decarboxylase (ADC) and ornithine decarboxylase (ODC). Hence, in this study, we generated independent ADC and ODC transgenic silenced tomato lines (SilADC ... [+]
The biosynthesis of putrescine is mainly driven by arginine decarboxylase (ADC) and ornithine decarboxylase (ODC). Hence, in this study, we generated independent ADC and ODC transgenic silenced tomato lines (SilADC and SilODC, respectively) to test the effect of defective ADC and ODC gene expression on root development under nitrate (NN) or ammonium (NA) conditions. The results showed that SilODC seedlings displayed an increase in ADC expression that led to polyamine accumulation, suggesting a compensatory effect of ADC. However, this effect was not observed in SilADC seedlings. These pathways are involved in different growth processes. The SilADC seedlings showed an increase in fresh weight, shoot length, lateral root number and shoot:root ratio under the NN source and an enhancement in fresh weight, and shoot and root length under NA conditions. However, SilODC seedlings displayed greater weight and shoot length under the NN source, whereas a decrease in lateral root density was found under NA conditions. Moreover, two overexpressed ODC lines were generated to check the relevance of the compensatory effect of the ADC pathway when ODC was silenced. These overexpressed lines showed not only an enhancement of almost all the studied growth parameters under both N sources but also an amelioration of ammonium syndrome under NA conditions. Together, these results reflect the importance of both pathways in plant growth, particularly ODC silencing, which requires compensation by ADC induction. [-]
Publicado en
Journal of Plant Physiology 268 (2022) 153560Datos relacionados
https://www.sciencedirect.com/science/article/pii/S0176161721001991#appsec1Entidad financiadora
Universitat Jaume I | Ministerio de Ciencia, Innovación y Universidades (Spain)
Código del proyecto o subvención
UJI-A2016-09 | UJI-B2020-24 | AGL2017-85987-C3-1-R | UJI-PREDOC/2016/27
Derechos de acceso
0176-1617/© 2021 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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