Development and validation of a QuEChERS-like method coupled to UHPLC-MS/MS for the determination of tobacco-specific toxicants in silicone personal samplers
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Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/158176
comunitat-uji-handle2:10234/71345
comunitat-uji-handle3:10234/73147
comunitat-uji-handle4:
TFG-TFMMetadatos
Título
Development and validation of a QuEChERS-like method coupled to UHPLC-MS/MS for the determination of tobacco-specific toxicants in silicone personal samplersAutoría
Tutor/Supervisor; Universidad.Departamento
Portolés Nicolau, Tania; Universitat Jaume I. Departament de Química Física i Analítica; Ramírez González, NoeliaFecha de publicación
2021-09-10Editor
Universitat Jaume IResumen
Air pollution is currently a problem that affects all living species in the globe and a ubiquitous group of compounds is rising concern about their ubiquitous presence in the atmosphere. These are toxicants derived ... [+]
Air pollution is currently a problem that affects all living species in the globe and a ubiquitous group of compounds is rising concern about their ubiquitous presence in the atmosphere. These are toxicants derived from tobacco consumption worldwide and humans are exposed to them constantly and involuntarily. This exposure can be studied through the thirdhand smoke (THS), which is the residual tobacco present in the air and surfaces after a cigarette has been smoked. This determination is conducted to urine samples but recently, silicone wristbands have been introduced as effective personal samplers as they can absorb a large number of compounds, which later are extracted and analysed to provide the amount of pollutants to which the person has been exposed during a period of time. This work purposes a method to determine simultaneously nicotine and five derivatives that are absorbed in a silicone wristband worn seven days as a personal passive sampler. For the optimization purposed, several optimization processes have been performed to reach to a method based on a QuEChERS extraction of the wristbands followed by a HPLC-MS/MS analysis. All compounds showed a good linearity whilst the extraction recoveries obtained ranged from 71-115% and the repeatability between 6-15%. As part of the validation process, two samples were analysed one being a non-smoker/non-exposed person and another being a smoker/exposed. Applying the method, it was able to identify all the target compounds in the smoker sample with levels of 2031ng·g-1 WB of nicotine, otherwise only nicotine and cotinine were detected in the non-smoker. With this it was proved the existence of ubiquitous tobaccospecific toxicants in the environment. [-]
Palabras clave / Materias
Descripción
Treball Final de Màster Universitari en Tècniques Cromatogràfiques Aplicades (Pla de 2013). Codi: SIY009
Curs acadèmic: 2020-2021
Tipo de documento
info:eu-repo/semantics/masterThesisDerechos de acceso
info:eu-repo/semantics/openAccess