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dc.contributor.authorCorreia, Barbara
dc.contributor.authorHancock, Robert D.
dc.contributor.authorAmaral, Joana
dc.contributor.authorGómez Cadenas, Aurelio
dc.contributor.authorValledor, Luis
dc.contributor.authorPinto, Glória
dc.identifier.citationCORREIA, Barbara; HANCOCK, Robert D.; AMARAL, Joana; GÓMEZ CADENAS, Aurelio; VALLEDOR, Luis; PINTO, Glória (2018). Combined Drought and Heat Activates Protective Responses in Eucalyptus globulus That Are Not Activated When Subjected to Drought or Heat Stress Alone. Frontiers in Plant Science, v. 9ca_CA
dc.description.abstractAiming to mimic a more realistic field condition and to determine convergent and divergent responses of individual stresses in relation to their combination, we explored physiological, biochemical, and metabolomic alterations after drought and heat stress imposition (alone and combined) and recovery, using a drought-tolerant Eucalyptus globulus clone. When plants were exposed to drought alone, the main responses included reduced pre-dawn water potential ( 9 pd ) and gas exchange. This was accompanied by increases in malondialdehyde (MDA) and total glutathione, indicative of oxidative stress. Abscisic acid (ABA) levels increased while the content of jasmonic acid (JA) fell. Metabolic alterations included reductions in the levels of sugar phosphates accompanied by increases in starch and non-structural carbohydrates. Levels of α -glycerophosphate and shikimate were also reduced while free amino acids increased. On the other hand, heat alone triggered an increase in relative water content (RWC) and 9 pd . Photosynthetic rate and pigments were reduced accompanied by a reduction in water use efficiency. Heat-induced a reduction of salicylic acid (SA) and JA content. Sugar alcohols and several amino acids were enhanced by the heat treatment while starch, fructose-6-phosphate, glucose-6-phosphate, and α -glycerophosphate were reduced. Contrary to what was observed under drought, heat stress activated the shikimic acid pathway. Drought-stressed plants subject to a heat shock exhibited a sharp decrease in gas exchange, 9 pd and JA, no alterations in electrolyte leakage, MDA, starch, and pigments and increased glutathione pool in relation to control. Comparing this with drought stress alone, subjecting drought stressed plants to an additional heat stress alleviated 9 pd and MDA, maintained an increased glutathione pool and reduced starch content and non-structural carbohydrates. A novel response triggered by the combined stress was the accumulation of cinnamate. Regarding recovery, most of the parameters affected by each stress condition reversed after re-establishment of control growing conditions. These results highlight that the combination of drought andca_CA
dc.format.extent14 p.ca_CA
dc.publisherFrontiers Mediaca_CA
dc.relation.isPartOfFrontiers in Plant Science (2018), v. 9ca_CA
dc.rightsAtribución 4.0 Internacional*
dc.subjectPlant metabolismca_CA
dc.subjectIsolated stressca_CA
dc.subjectCombined stressca_CA
dc.subjectNetwork analysisca_CA
dc.titleCombined Drought and Heat Activates Protective Responses in Eucalyptus globulus That Are Not Activated When Subjected to Drought or Heat Stress Aloneca_CA
dc.relation.projectID1) FEDER within the PT2020 Partnership Agreement and Compete 2020 (Programa Operacional Fatores de Competitividade); 2) National Funds through the Portuguese Foundation for Science and Technology (FCT), which financed CESAM (UID/AMB/50017 - POCI-01-0145-FEDER-007638) and the project PTDC/AGR- CFL/112996/2009; 3) FCT also supported the fellowships of BC (SFRH/BD/86448/2012), JA (SFRH/BD/120967/2016), and GP (SFRH/BPD/101669/2014); 4) Ramón y Cajal Program (RYC-2015-17871) (Spanish Ministry of Economy and Competitiveness). Rural and Environmental Science and Analytical Services Division of the Scottish Government.ca_CA
dc.contributor.funderRural and Environmental Science and Analytical Services Division of the Scottish Governmentca_CA

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Atribución 4.0 Internacional
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