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Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECTin Guinea Pig: Role of Bitter Taste Agonists
dc.contributor.author | Ortiz, José L. | |
dc.contributor.author | Ortiz, Amparo | |
dc.contributor.author | MILARA, JAVIER | |
dc.contributor.author | Armengot, Miguel | |
dc.contributor.author | Sanz, Celia | |
dc.contributor.author | Compañ, Desamparados | |
dc.contributor.author | Morcillo, Esteban | |
dc.contributor.author | Cortijo, Julio | |
dc.date.accessioned | 2017-02-14T15:39:09Z | |
dc.date.available | 2017-02-14T15:39:09Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Ortiz JL, Ortiz A, Milara J, Armengot M, Sanz C, Compañ D, et al. (2016) Evaluation of Mucociliary Clearance by Three Dimension MicroCT-SPECT in Guinea Pig: Role of Bitter Taste Agonists. PLoS ONE 11(10): e0164399. doi:10.1371/journal.pone.0164399 | ca_CA |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10234/166072 | |
dc.description.abstract | Different image techniques have been used to analyze mucociliary clearance (MCC) in humans, but current small animal MCC analysis using in vivo imaging has not been well defined. Bitter taste receptor (T2R) agonists increase ciliary beat frequency (CBF) and cause bronchodilation but their effects in vivo are not well understood. This work analyzes in vivo nasal and bronchial MCC in guinea pig animals using three dimension (3D) microCT-SPECT images and evaluates the effect of T2R agonists. Intranasal macroaggreggates of albumin-Technetium 99 metastable (MAA-Tc99m) and lung nebulized Tc99m albumin nanocolloids were used to analyze the effect of T2R agonists on nasal and bronchial MCC respectively, using 3D micro-CT-SPECT in guinea pig. MAA-Tc99m showed a nasal mucociliary transport rate of 0.36 mm/min that was increased in presence of T2R agonist to 0.66 mm/min. Tc99m albumin nanocolloids were homogeneously distributed in the lung of guinea pig and cleared with time-dependence through the bronchi and trachea of guinea pig. T2R agonist increased bronchial MCC of Tc99m albumin nanocolloids. T2R agonists increased CBF in human nasal ciliated cells in vitro and induced bronchodilation in human bronchi ex vivo. In summary, T2R agonists increase MCC in vivo as assessed by 3D microCT-SPECT analysis. | ca_CA |
dc.description.sponsorShip | The study was supported by the spanish goverment grants SAF2014-55322-P (JC; https://sede.micinn.gob.es/stfls/eSede/Ficheros/2015/Anexo_Preseleccionados_PRP_Proyectos_Excelencia_2014.pdf), SAF2015-65368-R (EM; https://sede.micinn.gob.es/stfls/eSede/Ficheros/2016/Anexo_Preseleccionados_Propuesta_Resolucion_Provisional_Proyectos_Retos_2015.pdf), FIS CP11/00293, FIS PI14/01733 (JM; http://gesdoc.isciii.es/?action=download&id=08/10/2014-fe2a818ac5) and centro de investigaciones biomédicas en red de enfermedades respiratorias (CIBERES; CB06/06/0027; http://www.ciberes.org/), TRACE (TRA2009-0311; (https://www.boe.es/boe/dias/2010/08/05/pdfs/BOE-A-2010-12605.pdf) as well as by fondos de la Generalitat Valenciana Prometeo II/2013/014 (EM, JC, JM; http://www.cece.gva.es/poci/docs/2013/NUEVAS/RESOLUCIONES/PROMETEOII13_Resolucion_Concesion_c.pdf). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ca_CA |
dc.format.extent | 17 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Public Library of Science | ca_CA |
dc.relation.isPartOf | PLoS ONE, October 10, 2016 | ca_CA |
dc.rights | © 2016 Ortiz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ca_CA |
dc.rights | Atribución 4.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-sa/4.0/ | * |
dc.title | Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECTin Guinea Pig: Role of Bitter Taste Agonists | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0164399 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164399 | ca_CA |
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