Comparison of approaches to deal with matrix effects in LC-MS/MS based determinations of mycotoxins in food and feed
Ver/ Abrir
Impacto
Scholar |
Otros documentos de la autoría: Fabregat-Cabello, Neus; Zomer, P.; Sancho, Juan V; Roig-Navarro, Antoni F.; Mol, Johannes G. J.
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/33596
comunitat-uji-handle3:10234/33597
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
Comparison of approaches to deal with matrix effects in LC-MS/MS based determinations of mycotoxins in food and feedAutoría
Fecha de publicación
2016-03xmlui.dri2xhtml.METS-1.0.item-edition
Versió post-print de l'autorEditor
© 2015 Wageningen Academic PublishersCita bibliográfica
FABREGAT-CABELLO, N., et al. Comparison of approaches to deal with matrix effects in LC-MS/MS based determinations of mycotoxins in food and feed. World Mycotoxin Journal, 2015, 1-14.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://www.wageningenacademic.com/doi/abs/10.3920/WMJ2014.1872Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
This study deals with one of the major concerns in mycotoxin determinations: the matrix effect related to LC-MS/ MS systems with electrospray ionization sources. To this end, in a first approach, the matrix effect has ... [+]
This study deals with one of the major concerns in mycotoxin determinations: the matrix effect related to LC-MS/ MS systems with electrospray ionization sources. To this end, in a first approach, the matrix effect has been evaluated in two ways: monitoring the signal of a compound (added to the mobile phase) during the entire chromatographicrun, and by classical post-extraction addition. The study was focused on nine selected mycotoxins: aflatoxin B1, fumonisins B1, B2 and B3, ochratoxin A, deoxynivalenol, T-2 and HT-2 toxins and zearalenone in various sample extracts giving moderate to strong matrix effects (maize, compound feed, straw, spices). Although the permanent monitoring of a compound provided a qualitative way of evaluating the matrix effects at each retention time, we concluded that it was not adequate as a quantitative approach to correct for the matrix effect. Matrix effects measured by post-extraction addition showed that the strongest ion suppression occurred for the spices (up to -89%). Five different calibration approaches to compensate for matrix effects were compared: multi-level external calibration using isotopically labelled internal standards, multi-level and single level standard addition, and two ways of singlepoint internal calibration: one point isotopic internal calibration and isotope pattern deconvolution. In general, recoveries and precision meeting the European Union requirements could be achieved with all approaches, with the exception of the single level standard addition at levels too close to the concentration in the sample. When an isotopically labelled internal standard is not available, single-level standard addition is the most efficient option. [-]
Publicado en
World Mycotoxin Journal, 2016; 9 (2): 149-161Derechos de acceso
© 2015 Wageningen Academic Publishers
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- IUPA_Articles [307]