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dc.contributor.authorPeris Vicente, Juan
dc.contributor.authorVillarreal Traver, Mónica
dc.contributor.authorCasas Breva, Inmaculada
dc.contributor.authorCarda-Broch, Samuel
dc.contributor.authorEsteve-Romero, Josep
dc.identifier.citationPERIS‐VICENTE, Juan, et al. Use of micellar liquid chromatography to analyze darunavir, ritonavir, emtricitabine, and tenofovir in plasma. Journal of separation science, 2014, 37.20: 2825-2832.ca_CA
dc.description.abstractDanuravir, ritonavir, emtricitabine, and tenofovir are together prescribed against AIDS as a highly active antiretroviral therapy regimen. Micellar liquid chromatography has been applied to determine these four antiretroviral drugs in plasma. The sample preparation is shortened to the dilution of the sample in a micellar solution, filtration, and injection. Clean-up steps are avoided, due to the solubilization of plasma matrix in micellar media. The drugs were analyzed in <20 min using a mobile phase of 0.06 M sodium dodecyl sulfate/2.5% 1-pentanol (pH 7) running under isocratic mode through a C18 column at 1 mL/min at room temperature. Absorbance wavelength detection was set at 214 nm. The method was successfully validated following the ICH Harmonized Tripartite Guideline in terms of selectivity, limit of detection (0.080–0.110 μg/mL), limit of quantification (0.240–0.270 μg/mL), linearity between 0.25 and 25 μg/mL (r2 > 0.995), accuracy (89.3–103.2%), precision (<8.2%) and robustness (<7.5%). Real plasma sample from patients taking this therapy were analyzed. This is the first paper showing the simultaneous detection of this four drugs. Therefore, the methodology was proven useful for the routine analysis of these samples in a hospital laboratory for clinical purposes.ca_CA
dc.format.extent7 p.ca_CA
dc.relation.isPartOfJournal of Separation Science Volume 37, Issue 20, October 2014ca_CA
dc.rightsCopyright © 1999-2015 John Wiley & Sons, Inc. All Rights Reserved.ca_CA
dc.titleUse of micellar liquid chromatography to analyze darunavir, ritonavir, emtricitabine, and tenofovir in plasmaca_CA

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