Development and validation of a micellar liquid chromatography-based method to quantify melamine in swine kidney
Impacte
Scholar |
Altres documents de l'autoria: Beltrán Martinavarro, Beatriz; Vives-Peris, Vicente; Carda-Broch, Samuel; Esteve-Romero, Josep
Metadades
Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
comunitat-uji-handle4:
INVESTIGACIONAquest recurs és restringit
http://dx.doi.org/10.1016/j.foodcont.2014.05.027 |
Metadades
Títol
Development and validation of a micellar liquid chromatography-based method to quantify melamine in swine kidneyAutoria
Data de publicació
2014Editor
ElsevierISSN
0956-7135Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://www.sciencedirect.com/science/article/pii/S0956713514002734Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Melamine is a toxic compound illegally added to animal feed to falsely boost protein content. This represents a strong threat to consumer's health, as melamine can reach human through the food chain. An easy and ... [+]
Melamine is a toxic compound illegally added to animal feed to falsely boost protein content. This represents a strong threat to consumer's health, as melamine can reach human through the food chain. An easy and reliable micellar liquid chromatography-method was developed to detect melamine in swine kidney. The analyte was extracted by shaking in methanol. Melamine was eluted from the HPLC column without interferences in <8.0 min. Mobile phase was a 0.11 M sodium dodecyl sulfate – 7.5% propanol at pH 3 solution running under isocratic mode through a C18 column at 1 mL/min and absorbance detection at 210 nm. The method was validated according to the Food and Drug Administration guidelines: sensitivity, calibration range (0.3–30 μg/g), linearity (r2 > 0.9997), precision (<7.6%), accuracy (−8.3–3.6%), recovery (82.1–92.4%) and robustness (<5.1%). The methodology was applied to swine kidney samples purchased from a local supermarket. The method can be used as a screening method for routine post-mortem diagnose melamine intoxication in animals and detect feeding with poultry containing melamine, in order to withdraw the corresponding flesh. This would be mandatory to prevent consumer intoxication and improve the quality of food. [-]
Publicat a
Food Control, 2014, vol. 46, p. 168-173.Drets d'accés
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
Apareix a les col.leccions
- QFA_Articles [829]