Computational study of the mechanism of half-reactions in class 1A dihydroorotate dehydrogenase from Trypanosoma cruzi
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Other documents of the author: De Farias Silva, Natália; Lameira, Jerônimo; Nahum Alves, Cláudio; Martí Forés, Sergio
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http://dx.doi.org/10.1039/c3cp52692e |
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Title
Computational study of the mechanism of half-reactions in class 1A dihydroorotate dehydrogenase from Trypanosoma cruziDate
2013Publisher
Royal Society of ChemistryISSN
1463-9076; 1463-9084Bibliographic citation
Phys. Chem. Chem. Phys.,2013, 15, 18863Type
info:eu-repo/semantics/articlePublisher version
http://pubs.rsc.org/en/content/articlepdf/2013/cp/c3cp52692eVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Chagas' disease is considered to be a health problem affecting millions of people in Latin America. This disease is caused by the parasite Trypanosoma cruzi. Recently dihydroorotate dehydrogenase class 1A from Trypa ... [+]
Chagas' disease is considered to be a health problem affecting millions of people in Latin America. This disease is caused by the parasite Trypanosoma cruzi. Recently dihydroorotate dehydrogenase class 1A from Trypanosoma cruzi (TcDHODA) was shown to be essential for the survival and growth of T. cruzi and proposed as a drug target against Chagas' disease. This enzyme catalyzes the oxidation of (S)-dihydroorotate to orotate, with a proposed catalytic cycle consisting of two half-reactions. In the first half-reaction dihydroorotate is oxidized to orotate, with the consequent reduction of the flavin mononucleotide cofactor. In the second half-reaction fumarate is reduced to succinate. The first oxidation half-reaction may occur via a concerted or a stepwise mechanism. Herein, the catalytic mechanism of TcDHODA has been studied using hybrid Quantum Mechanical/Molecular Mechanical (QM/MM) Molecular Dynamics (MD) simulations. The free energy profiles derived from the bidimensional potential of mean force reveal more details for two half-reaction processes. [-]
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Physical Chemistry Chemical Physics, 2013, Volume 15, Issue 43Rights
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