Toward an Automatic Determination of Enzymatic Reaction Mechanisms and Their Activation Free Energies
comunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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http://dx.doi.org/ 10.1021/ct400153r |
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Title
Toward an Automatic Determination of Enzymatic Reaction Mechanisms and Their Activation Free EnergiesDate
2013Publisher
American Chemical SocietyISSN
1549-9618Bibliographic citation
ZINOVJEV, Kirill; RUIZ-PERNÍA, J. Javier; TUÑÓN, Iñaki. Toward an automatic determination of enzymatic reaction mechanisms and their activation free energies. Journal of Chemical Theory and Computation, 2013, 9.8: 3740-3749.Type
info:eu-repo/semantics/articlePublisher version
http://pubs.acs.org/doi/abs/10.1021/ct400153rVersion
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Abstract
We present a combination of the string method and a path collective variable for the exploration of the free energy surface associated to a chemical reaction in condensed environments. The on-the-fly string method is ... [+]
We present a combination of the string method and a path collective variable for the exploration of the free energy surface associated to a chemical reaction in condensed environments. The on-the-fly string method is employed to find the minimum free energy paths on a multidimensional free energy surface defined in terms of interatomic distances, which is a convenient selection to study bond forming/breaking processes. Once the paths have been determined, a reaction coordinate is defined as a measure of the advance of the system along these paths. This reaction coordinate can be then used to trace the reaction Potential of Mean Force from which the activation free energy can be obtained. This combination of methodologies has been here applied to the study, by means of Quantum Mechanics/Molecular Mechanics simulations, of the reaction catalyzed by guanidinoacetate methyltransferase. This enzyme catalyzes the methylation of guanidinoacetate by S-adenosyl-l-methionine, a reaction that involves a methyl transfer and a proton transfer and for which different reaction mechanisms have been proposed. [-]
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Journal of Chemical Theory and Computation, 2013, 9.8Rights
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