Dispersion management in two-photon microscopy by using diffractive optical elements
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Títol
Dispersion management in two-photon microscopy by using diffractive optical elementsAutoria
Data de publicació
2013Editor
Optical Society of AmericaISSN
0146-9592; 1539-4794Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://www.opticsinfobase.org/ol/fulltext.cfm?uri=ol-38-4-440&id=249076Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
We demonstrate efficient generation of wide-field fluorescence signals in two-photon microscopy exploiting diffractive optical elements and short pulses by using a dispersion-compensated beam delivery optics module. ... [+]
We demonstrate efficient generation of wide-field fluorescence signals in two-photon microscopy exploiting diffractive optical elements and short pulses by using a dispersion-compensated beam delivery optics module. Computer-generated holograms are codified onto a phase-only spatial light modulator, which allows for arbitrary single-shot patterning of the sample. Spatiotemporal shaping of the pulse is mandatory to overcome spatial chirp and pulse-front tilt effects that spread both in space and time the irradiance patterns, thus limiting not only the spatial resolution but also the signal-to-noise ratio in two-photon microscopy. By using a multipass amplifier delivering 30 fs, 0.8 mJ pulses at 1 kHz repetition rate, we experimentally demonstrated arbitrary single-shot fluorescence irradiance patterns in Rhodamine B. [-]
Publicat a
Optics letters, 2013, Vol. 38, No. 4Drets d'accés
© 2013 Optical Society of America. "This paper was published in OPTICS LETTERS and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/ol/fulltext.cfm?uri=ol-38-4-440&id=249076. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law".
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info:eu-repo/semantics/openAccess
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