Listar FCA_Articles por autoría "bda15c25-3736-490c-aa3f-1b2499e72a6b"
Mostrando ítems 1-20 de 48
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Access resistance in protein nanopores. A structure-based computational approach
Aguilella-Arzo, Marcel; Aguilella, Vicente Elsevier (2020-02)Single-channel conductance measurements in biological pores have demonstrated the importance of interfacial effects in nanopores, particularly in protein channels with low aspect ratio (length over aperture radius). Access ... -
Amphiphilic COSAN and I2-COSAN crossing synthetic lipid membranes: planar bilayers and liposomes
Verdiá Báguena, Carmen; Alcaraz, Antonio; Aguilella, Vicente; Cioran, Ana M.; Tachikawa, Shoji; Nakamura, Hiroyuki; Teixidor, Francesc; Viñas, Clara Royal Society of Chemistry (2014-03)The boron-rich cobaltabisdicarbollide (COSAN) and its 8,80-I2 derivative (I2-COSAN), both of purely inorganic nature, are shown to cross through synthetic lipid membranes. These results reveal unexpected properties at the ... -
Analysis of SARS-CoV E protein ion channel activity by tuning the protein and lipid charge
Verdiá Báguena, Carmen; Nieto Torres, José L.; Alcaraz, Antonio; De Diego, Marta L.; Enjuanes, Luis; Aguilella, Vicente Elsevier (2013-05-18)A partial characterization of the ion channels formed by the SARS coronavirus (CoV) envelope (E) protein was previously reported (C. Verdiá-Báguena et al., 2012 [12]). Here, we provide new significant insights on the ... -
Beyond the energy balance: Exergy analysis of an industrial roller kiln firing porcelain tiles
Ferrer Castán, Salvador; Mezquita, Ana; Aguilella, Vicente; Monfort, Eliseo Elsevier (2019-03)The ceramic tile manufacturing process consumes large amounts of energy, mainly in the firing stage. Firing usually takes place in natural gas-fuelled continuous roller kilns, the most widely used tile firing facilities ... -
Biphasic concentration patterns in ionic transport under nanoconfinement revealed in steady-state and time-dependent properties
Queralt-Martín, María; Perez-Grau, Jose J.; Alvero González, Laidy Maidel; Perini, Deborah Aurora; Cervera, Javier; Aguilella, Vicente; Alcaraz, Antonio American Institute of Physics (2023-02-08)Ion permeation across nanoscopic structures differs considerably from microfluidics because of strong steric constraints, transformed solvent properties, and charge-regulation effects revealed mostly in diluted solutions. ... -
Boron clusters (ferrabisdicarbollides) shaping the future as radiosensitizers for multimodal (chemo/radio/PBFR) therapy of glioblastoma
Nuez Martínez, Miquel; Queralt-Martín, María; Aguilella, Vicente; VV.AA. Royal Society of Chemistry (2022-10-24)Glioblastoma multiforme (GBM) is the most common and fatal primary brain tumor, and is highly resistant to conventional radiotherapy and chemotherapy. Therefore, the development of multidrug resistance and tumor recurrence ... -
Channel-Inactivating Mutations and Their Revertant Mutants in the Envelope Protein of Infectious Bronchitis Virus
To, Janet; Surya, Wahyu; To, Sing Fung; Li, Yan; Verdiá Báguena, Carmen; Queralt-Martín, María; Aguilella, Vicente; Ding, Xiang Liu; Torres, Jaume American Society for Microbiology (2016-12)It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity ... -
Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure
Aguilella-Arzo, Marcel; Hoogerheide, David; Doucet, Mathieu; Wang, Hanyu; Aguilella, Vicente American Chemical Society (2024-01-31)Macromolecular crowding is the usual condition of cells. The implications of the crowded cellular environment for protein stability and folding, protein–protein interactions, and intracellular transport drive a growing ... -
Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure
Aguilella-Arzo, Marcel; Hoogerheide, David; Doucet, Mathieu; Wang, Hanyu; Aguilella, Vicente American Chemical Society (2024-01-16)Macromolecular crowding is the usual condition of cells. The implications of the crowded cellular environment for protein stability and folding, protein-protein interactions, and intracellular transport drive a growing ... -
Coronavirus E protein forms ion channels with functionally and structurally-involved membrane lipids
Aguilella, Vicente; Alcaraz, Antonio; Verdiá Báguena, Carmen; Nieto Torres, José L.; De Diego, Marta L.; Torres, Jaume; Enjuanes, Luis Elsevier (2012-10)Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV ... -
Dielectric saturation of water in a membrane protein channel
Aguilella-Arzo, Marcel; Andrio, Andreu; Aguilella, Vicente; Alcaraz, Antonio Royal Society of Chemistry (2009)Water molecules in confined geometries like nanopores and biological ion channels exhibit structural and dynamical properties very different from those found in free solution. Protein channels that open aqueous pores ... -
Diffusion, exclusion, and specific binding in a large channel: A study of OmpF selectivity inversion
Alcaraz, Antonio; Nestorovich E.M.; López Peris, María Lidón; García Giménez, Elena; Bezrukov S.M.; Aguilella, Vicente Elsevier (2009)We find that moderate cationic selectivity of the general bacterial porin OmpF in sodium and potassium chloride solutions is inversed to anionic selectivity in concentrated solutions of barium, calcium, nickel, and magnesium ... -
Directional ion selectivity in a biological nanopore with bipolar structure
García Giménez, Elena; Alcaraz, Antonio; Aguilella, Vicente; Ramírez, Patricio Elsevier (2009)Ion transport features of a biological nanopore, the bacterial porin OmpF from Escherichia coli, have been investigated by patch-clamp experiments performed at the single channel level. Membrane potential measurements done ... -
Divalent Metal Ion Transport across Large Biological Ion Channels and Their Effect on Conductance and Selectivity
García Giménez, Elena; Alcaraz, Antonio; Aguilella, Vicente Hindawi Publishing Corporation (2012-07)Electrophysiological characterization of large protein channels, usually displaying multi-ionic transport and weak ion selectivity, is commonly performed at physiological conditions (moderate gradients of KCl solutions at ... -
Effect of the Endosomal Acidification on Small Ion Transport through the Anthrax Toxin PA63 Channel
Kalu, Nnanya; Alcaraz, Antonio; Yamini, Goli; Mombem Abolfath, Sanaz; Lucas, Laura; Kenney, Clare; Aguilella, Vicente; Nestorovich, Ekaterina M. Wiley (2017-11)Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is ‘hijacked’ by ... -
Electrical pumping of potassium ions against an external concentration gradient in a biological ion channel
Aguilella, Vicente; Alcaraz, Antonio; Ramírez, Patricio; García Giménez, Elena; Mafe, Salvador; Queralt-Martín, María AIP Publishing (2013-07-22)We show experimentally and theoretically that significant currents can be obtained with a biological ion channel, the OmpF porin of Escherichia coli, using zero-average potentials as driving forces. The channel rectifying ... -
Electrokinetic phenomena in microporous membranes with a fixed transverse charge distribution
Aguilella, Vicente; Aguilella-Arzo, Marcel; Ramírez, Patricio Elsevier (1996-05)The charged capillary model for microporous membranes has been extended to account for a distribution of fixed charge transversal to the pore wall. The non-linearized Poisson-Boltzmann equation has been solved numerically ... -
Entropy–enthalpy compensation at the single protein level: pH sensing in the bacterial channel OmpF
Alcaraz, Antonio; Queralt-Martín, María; Verdiá Báguena, Carmen; Aguilella, Vicente; Mafe, Salvador The Royal Society of Chemistry (2014)The pH sensing mechanism of the OmpF channel operates via ligand modification: increasing acidity induces the replacement of cations with protons in critical binding sites decreasing the channel conductance. Aside from the ... -
Hydrophobic Pulmonary Surfactant Proteins SP-B and SP-C Induce Pore Formation in Planar Lipid Membranes: Evidence for Proteolipid Pores
Parra, Elisa; Alcaraz, Antonio; Cruz, Antonio; Aguilella, Vicente; Pérez Gil, Jesús Biophysical Society (2013-01-08)Pulmonary surfactant is a complex mixture of lipids and specific surfactant proteins, including the hydrophobic proteins SP-B and SP-C, in charge of stabilizing the respiratory surface of mammalian lungs. The combined ... -
Increased salt concentration promotes competitive block of OmpF channel by protons
Alcaraz, Antonio; García Giménez, Elena; Aguilella, Vicente; Queralt-Martín, María Elsevier (2012-11)Porins are channel-forming proteins that are located in the outer membranes (OM) of Gram-negative bacteria and allow the influx of hydrophilic nutrients and the extrusion of waste products. The fine regulation of the ion ...