Detection, characterization and quantification of salicylic acid conjugates in plant extracts by ESI tandem mass spectrometric techniques
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Other documents of the author: Pastor, Victoria; Vicent Barrera, Cristian; Cerezo García, Miguel; Mauch-Mani, Brigitte; Dean, John; Flors, Victor
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http://dx.doi.org/10.1016/j.plaphy.2012.01.003 |
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Title
Detection, characterization and quantification of salicylic acid conjugates in plant extracts by ESI tandem mass spectrometric techniquesAuthor (s)
Date
2012Publisher
ElsevierISSN
0981-9428; 1873-2690Type
info:eu-repo/semantics/articlePublisher version
http://ac.els-cdn.com/S0981942812000058/1-s2.0-S0981942812000058-main.pdf?_tid=4 ...Subject
Abstract
An approach for the detection and characterization of SA derivatives in plant samples is presented based
on liquid chromatography coupled to electrospray ionization (ESI) tandem mass spectrometric techniques. Precursor ... [+]
An approach for the detection and characterization of SA derivatives in plant samples is presented based
on liquid chromatography coupled to electrospray ionization (ESI) tandem mass spectrometric techniques. Precursor ion scan methods using an ESI triple quadrupole spectrometer for samples from plants
challenged with the virulent Pseudomonas syringae pv tomato DC3000 allowed us to detect two potential
SA derivatives. The criterion used to consider a potential SA derivative is based on the detection of
analytes in the precursor ion scan chromatogram upon selecting m/z 137 and m/z 93 that correspond to
the salicylate and its main product ion, respectively. Product ion spectra of the newly-detected analytes
as well as accurate m/z determinations using an ESI Q-time-of-flight instrument were registered as
means of characterization and strongly suggest that glucosylated forms of SA at the carboxylic and at the
phenol functional groups are present in plant samples. The specific synthesis and subsequent chromatography of salicylic glucosyl ester (SGE) and glucosyl salicylate (SAG) standards confirmed the chemical
identity of both peaks that were obtained applying different tandem mass spectrometric techniques and
accurate m/z determinations. A multiple reaction monitoring method has been developed and applied to
plant samples. The advantages of this LCeESIeMS/MS methods with respect to the traditional analysis of
glucosyl conjugates are also discussed. Preliminary results revealed that SA and the glucosyl conjugates
are accumulated in Arabidopsis thaliana in a time dependent manner, accordingly to the up-regulation of
SA-dependent defenses following P. syringae infection. This technique applied to plant hormones or
fragment ions may be useful to obtain chemical family members of plant metabolites and help identify
their contribution in the signaling of plant defenses [-]
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Plant Physiology and Biochemistry 53 (2012)Rights
© 2012 Elsevier Masson SAS. All rights reserved.
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