Amino acid tissue levels and GABAa receptor binding in the developing rat cerebellum following status epilepticus
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Other documents of the author: MIQUEL, MARTA; López Meraz, María Leonor; Rocha, Luisa L.; Ortega, Jenny C.; Pérez-Estudillo, César A.; García, Luis I.; Hernández, María Elena; Manzo, Jorge
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comunitat-uji-handle2:10234/8033
comunitat-uji-handle3:10234/8636
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Title
Amino acid tissue levels and GABAa receptor binding in the developing rat cerebellum following status epilepticusAuthor (s)
Date
2012Publisher
ElsevierISSN
0006-8993Bibliographic citation
López-Meraz, M.-L., et al., Amino acid tissue levels and GABAA receptor binding in the developing rat cerebellum following status epilepticus, Brain Res. (2012), doi:10.1016/j.brainres.2011.12.038Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S000689931102289XVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Incidence of status epilepticus (SE) is higher in children than in adults and SE can be induced
in developing rats. The cerebellum can be affected after SE; however, consequences of
cerebellar amino acid transmission ... [+]
Incidence of status epilepticus (SE) is higher in children than in adults and SE can be induced
in developing rats. The cerebellum can be affected after SE; however, consequences of
cerebellar amino acid transmission have been poorly studied. The goal of this study was
to determine amino acid tissue concentration and GABAA receptor binding in the
immature rat cerebellum after an episode of SE. Thirteen-day-old (P13) rat pups received in-
traperitoneal injections of lithium chloride (3 mEq/kg). Twenty hours later, on P14, SE was
induced by subcutaneous injection of pilocarpine hydrochloride (60 mg/kg). Control ani-
mals were given an equal volume of saline subcutaneously. Animals were killed 24 h after
SE induction, the cerebellum was quickly removed, and the vermis and hemispheres were
rapidly dissected out on ice. Amino acid tissue concentrations in the vermis and hemi-
spheres were evaluated by HPLC and fluorescent detection. GABAA receptor binding in the
medial vermis was analyzed by in vitro autoradiography. SE increased the tissue levels of
the inhibitory amino acids taurine (80%) and alanine (91%), as well as glutamine (168%) in
the cerebellar hemisphere; no changes were observed in the vermis. SE did not modify
GABAA receptor binding in any cerebellar lobule from the vermis. Our data demonstrate
that SE produces region-specific changes in amino acid concentrations in the developing
cerebellum. [-]
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