Quinolones control in milk and eggs samples by liquid chromatography using a surfactant-mediated mobile phase
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comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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http://dx.doi.org/10.1007/s00216-010-4409-x |
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Title
Quinolones control in milk and eggs samples by liquid chromatography using a surfactant-mediated mobile phaseAuthor (s)
Date
2011Publisher
Springer-VerlagISSN
1618-2642Bibliographic citation
Analytical and Bioanalytical Chemistry (2011), 400, 5, p. 1303-1313Type
info:eu-repo/semantics/articlePublisher version
http://link.springer.com/article/10.1007/s00216-010-4409-xVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Four quinolones (danofloxacin, difloxacin, flumequine and marbofloxacin) were determined in milk and egg samples by a simplified high-performance liquid chromatographic procedure using a micellar mobile phase. No ... [+]
Four quinolones (danofloxacin, difloxacin, flumequine and marbofloxacin) were determined in milk and egg samples by a simplified high-performance liquid chromatographic procedure using a micellar mobile phase. No extraction was needed to precipitate the proteins from the matrices since they were solubilised in micelles. The only pretreatment steps required were homogenisation, dilution and filtration before injecting the sample into the chromatographic system. An adequate resolution of the quinolones was achieved by a chemometrics approach where retention was modelled as a first step using the retention factors in only five mobile phases. Afterwards, an optimisation criterion was applied to consider the position and shape of the chromatographic peaks. Analytical separation involved a C18 reversed-phase column, a hybrid micellar mobile phase of 0.05 M sodium dodecyl sulphate, 10% (v/v) butanol and 0.5% (v/v) triethylamine buffered at pH 3 and fluorimetric detection. Quinolones were eluted in less than 15 min without the protein band or other endogenous compounds from the food matrices interfering. The calculated relevant validation parameters, e.g., decision limit (CCα), detection capability (CCβ), repeatability, within-laboratory reproducibility, recoveries and robustness, were acceptable and complied with European Commission Decision 2002/657/EC. Finally, the proposed method was successfully employed in quantifying the four quinolones in spiked egg and milk samples. [-]
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