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Single tube optimisation of APOE genotyping based on melting curve analysis

dc.contributor.authorFrancés, F.
dc.contributor.authorPortolés, Olga
dc.contributor.authorSorlí, José V
dc.contributor.authorGuillen, M.
dc.contributor.authorGonzález, José I.
dc.contributor.authorCorella, Dolores
dc.date.accessioned2012-05-28T14:36:32Z
dc.date.available2012-05-28T14:36:32Z
dc.date.issued2008
dc.identifierhttp://dx.doi.org/10.1016/j.clinbiochem.2008.03.010
dc.identifier.citationClinical Biochemistry, 41, p. 923-926
dc.identifier.issn99120
dc.identifier.urihttp://hdl.handle.net/10234/38956
dc.description.abstractObjectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions. Results: Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol. Conclusions: This method is cheaper, faster and more reliable and versatile than other alternatives proposed. © 2008 Elsevier Inc. All rights reserved.
dc.language.isoeng
dc.publisherElsevier
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/*
dc.subjectAnalysis
dc.subjectGenotype
dc.subjectValidation studies
dc.titleSingle tube optimisation of APOE genotyping based on melting curve analysis
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doihttp://dx.doi.org/10.1016/j.clinbiochem.2008.03.010
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess


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