Single tube optimisation of APOE genotyping based on melting curve analysis
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7038
comunitat-uji-handle3:10234/8634
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http://dx.doi.org/10.1016/j.clinbiochem.2008.03.010 |
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Title
Single tube optimisation of APOE genotyping based on melting curve analysisAuthor (s)
Date
2008Publisher
ElsevierISSN
99120Bibliographic citation
Clinical Biochemistry, 41, p. 923-926Type
info:eu-repo/semantics/articleSubject
Abstract
Objectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that ... [+]
Objectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions. Results: Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol. Conclusions: This method is cheaper, faster and more reliable and versatile than other alternatives proposed. © 2008 Elsevier Inc. All rights reserved. [-]
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info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
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