Single tube optimisation of APOE genotyping based on melting curve analysis
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Scholar |
Altres documents de l'autoria: Francés, F.; Portolés, Olga; Sorlí, José V; Guillen, M.; González, José I.; Corella, Dolores
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http://dx.doi.org/10.1016/j.clinbiochem.2008.03.010 |
Metadades
Títol
Single tube optimisation of APOE genotyping based on melting curve analysisAutoria
Data de publicació
2008Editor
ElsevierISSN
99120Cita bibliogràfica
Clinical Biochemistry, 41, p. 923-926Tipus de document
info:eu-repo/semantics/articleParaules clau / Matèries
Resum
Objectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that ... [+]
Objectives: To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes. Design and methods: We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions. Results: Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol. Conclusions: This method is cheaper, faster and more reliable and versatile than other alternatives proposed. © 2008 Elsevier Inc. All rights reserved. [-]
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