Rapid degeneration of iPSC-derived motor neurons lacking Gdap1 engages a mitochondrial-sustained innate immune response
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Altres documents de l'autoria: León Rodríguez, Marian; Prieto, Javier; Molina Navarro, María Micaela; Garcia-Garcia, Francisco; Barneo-Muñoz, Manuela; Ponsoda, Xavier; Sáez, Rosana; Palau, Francesc; Dopazo, Joaquin; Izpisua Belmonte, Juan Carlos; Torres, Josema
Metadades
Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/36080
comunitat-uji-handle3:10234/36082
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INVESTIGACIONMetadades
Títol
Rapid degeneration of iPSC-derived motor neurons lacking Gdap1 engages a mitochondrial-sustained innate immune responseAutoria
Data de publicació
2023-07-01Editor
Springer NatureCita bibliogràfica
León, M., Prieto, J., Molina-Navarro, M.M. et al. Rapid degeneration of iPSC-derived motor neurons lacking Gdap1 engages a mitochondrial-sustained innate immune response. Cell Death Discov. 9, 217 (2023). https://doi.org/10.1038/s41420-023-01531-wTipus de document
info:eu-repo/semantics/articleVersió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Charcot-Marie-Tooth disease is a chronic hereditary motor and sensory polyneuropathy targeting Schwann cells and/or motor neurons. Its multifactorial and polygenic origin portrays a complex clinical phenotype of the ... [+]
Charcot-Marie-Tooth disease is a chronic hereditary motor and sensory polyneuropathy targeting Schwann cells and/or motor neurons. Its multifactorial and polygenic origin portrays a complex clinical phenotype of the disease with a wide range of genetic inheritance patterns. The disease-associated gene GDAP1 encodes for a mitochondrial outer membrane protein. Mouse and insect models with mutations in Gdap1 have reproduced several traits of the human disease. However, the precise function in the cell types affected by the disease remains unknown. Here, we use induced-pluripotent stem cells derived from a Gdap1 knockout mouse model to better understand the molecular and cellular phenotypes of the disease caused by the loss-of-function of this gene. Gdap1-null motor neurons display a fragile cell phenotype prone to early degeneration showing (1) altered mitochondrial morphology, with an increase in the fragmentation of these organelles, (2) activation of autophagy and mitophagy, (3) abnormal metabolism, characterized by a downregulation of Hexokinase 2 and ATP5b proteins, (4) increased reactive oxygen species and elevated mitochondrial membrane potential, and (5) increased innate immune response and p38 MAP kinase activation. Our data reveals the existence of an underlying Redox-inflammatory axis fueled by altered mitochondrial metabolism in the absence of Gdap1. As this biochemical axis encompasses a wide variety of druggable targets, our results may have implications for developing therapies using combinatorial pharmacological approaches and improving therefore human welfare. [-]
Entitat finançadora
IRDiRC | Instituto de Salud Carlos III | Fundación Alfonso Martín Escudero | Generalitat Valenciana
Codi del projecte o subvenció
IR11/TREAT-CMT | VALi+d 2019 program
Drets d'accés
© The Author(s) 2023
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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